A biochemical and immunological study of horseradish peroxidase

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dc.contributor.advisorSwart, P.
dc.contributor.advisorLombard, N.
dc.contributor.authorOdendaal, Ruenda
dc.contributor.otherUniversity of Stellenbosch. Faculty of Science. Dept. of Biochemistry.
dc.date.accessioned2009-11-24T06:29:22Zen_ZA
dc.date.accessioned2010-06-01T08:27:59Z
dc.date.available2009-11-24T06:29:22Zen_ZA
dc.date.available2010-06-01T08:27:59Z
dc.date.issued2009-12
dc.descriptionThesis (MSc (Biochemistry))--University of Stellenbosch, 2009.
dc.description.abstractENGLISH ABSTRACT: This study describes: a) the isolation and purification of horseradish peroxidase isoenzymes from horseradish roots, b) the characterization of various forms and components of the enzyme by cation-exchange and reversed-phase high performance liquid chromatography, c) the preparation of antibodies against horseradish peroxidase isoenzymes, d) immunological studies for the development of an isoenzyme quantification method and e) the formation of an enzyme-melamine conjugate for use in a melamine quantification immunoassay.en
dc.description.abstractAFRIKAANSE OPSOMMING: Hierdie studie beskryf: a) die isolering en suiwering van peperwortel-peroksidase-isoënsieme vanuit die peperwortel, b) die karakterisering van verskillende vorme en komponente van dié ensiem deur katioonuitruilings en omgekeerde-fase HPLC c) die voorbereiding van teenliggaampies vir peperwortel-peroksidase-isoënsieme, d) immunologiese studies vir die ontwikkeling van 'n isoënsiem-kwantifiseringsmetode; en e) die vorming van 'n ensiem-melamien-konjugaat vir gebruik in 'n melamienkwantifiseringsmetode.af
dc.identifier.urihttp://hdl.handle.net/10019.1/1587
dc.language.isoen
dc.publisherStellenbosch : University of Stellenbosch
dc.rights.holderUniversity of Stellenbosch
dc.subjectHorseradish peroxidaseen
dc.subjectIsoelectric focusingen
dc.subjectHPLCen
dc.subjectDissertations -- Biochemistryen
dc.subjectTheses -- Biochemistryen
dc.subject.lcshPeroxidase -- Analysisen
dc.subject.lcshIsoensymesen
dc.titleA biochemical and immunological study of horseradish peroxidaseen
dc.typeThesis
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