Microsatellite cross-species amplification and utility in southern African elasmobranchs : a valuable resource for fisheries management and conservation

Maduna, Simo N.; Rossouw, Charne; Roodt-Wilding, Rouvay; Bester-Van der Merwe, Aletta E.

Microsatellite cross-species amplification and utility in southern African elasmobranchs : a valuable resource for fisheries management and conservation

dc.contributor.author	Maduna, Simo N.	en_ZA
dc.contributor.author	Rossouw, Charne	en_ZA
dc.contributor.author	Roodt-Wilding, Rouvay	en_ZA
dc.contributor.author	Bester-Van der Merwe, Aletta E.	en_ZA
dc.date.accessioned	2014-07-28T09:48:28Z
dc.date.available	2014-07-28T09:48:28Z
dc.date.issued	2014
dc.date.updated	2014-07-02T19:03:54Z
dc.description	CITATION: Maduna, S. N., Rossouw, C., Roodt-Wilding, R. & Bester-Van Der Merwe, A. E. 2014. Microsatellite cross-species amplification and utility in southern African elasmobranchs: A valuable resource for fisheries management and conservation. BMC Research Notes, 7(1):352, doi:10.1186/1756-0500-7-352.	en_ZA
dc.description	The original publication is available at http://www.biomedcentral.com/1756-0500/7/352	en_ZA
dc.description.abstract	Background: Similarly to the rest of the world, southern Africa’s diverse chondrichthyan fauna is currently experiencing high fishing pressures from direct and non-direct fisheries to satisfy market demands for shark products such as fins and meat. In this study, the development of microsatellite markers through cross-species amplification of primer sets previously developed for closely related species is reported as an alternative approach to de novo marker development. This included the design of four microsatellite multiplex assays and their cross-species utility in genetic diversity analysis of southern African elasmobranchs. As this study forms part of a larger project on the development of genetic resources for commercially important and endemic southern African species, Mustelus mustelus was used as a candidate species for testing these multiplex assays in down-stream applications. Results Thirty five microsatellite primer sets previously developed for five elasmobranch species were selected from literature for testing cross-species amplification in 16 elasmobranch species occurring in southern Africa. Cross-species amplification success rates ranged from 28.6%-71.4%. From the successfully amplified microsatellites, 22 loci were selected and evaluated for levels of polymorphism, and four multiplex assays comprising of the 22 microsatellites were successfully constructed, optimised and characterised in a panel of 87 Mustelus mustelus individuals. A total of 125 alleles were observed across all loci, with the number of alleles ranging from 3–12 alleles. Cross-species amplification of the four optimised multiplex assays was further tested on 11 commercially important and endemic southern African elasmobranch species. Percentage of polymorphism ranged from 31.8%-95.5% in these species with polymorphic information content decreasing exponentially with evolutionary distance from the source species. Conclusions Cross-species amplification of the 35 microsatellites proved to be a time- and cost-effective approach to marker development in elasmobranchs and enabled the construction of four novel multiplex assays for characterising genetic diversity in a number of southern African elasmobranch species. This study successfully demonstrated the usefulness of these markers in down-stream applications such as genetic diversity assessment and species identification which could potentially aid in a more integrative, multidisciplinary approach to management and conservation of commercially important cosmopolitan and endemic elasmobranch species occurring in southern Africa.	en_ZA
dc.description.version	Publishers' Version	en_ZA
dc.format.extent	13 pages : illustrations	en_ZA
dc.identifier.citation	Maduna, S. N., Rossouw, C., Roodt-Wilding, R. & Bester-Van Der Merwe, A. E. 2014. Microsatellite cross-species amplification and utility in southern African elasmobranchs: A valuable resource for fisheries management and conservation. BMC Research Notes, 7(1):352, doi:10.1186/1756-0500-7-352.	en_ZA
dc.identifier.issn	1756-0500 (online)	en_ZA
dc.identifier.other	doi:10.1186/1756-0500-7-352	en_ZA
dc.identifier.uri	http://hdl.handle.net/10019.1/95516
dc.language.iso	en_ZA	en_ZA
dc.publisher	BMC Research Notes	en_ZA
dc.rights.holder	Simo N Maduna et al.; licensee BioMed Central Ltd.	en_ZA
dc.subject	Microsatellite (Genetics) amplification	en_ZA
dc.subject	Conservation management	en_ZA
dc.subject	Fisheries management	en_ZA
dc.subject	Chondrichthyes -- Southern African -- Genetic diversity	en_ZA
dc.title	Microsatellite cross-species amplification and utility in southern African elasmobranchs : a valuable resource for fisheries management and conservation	en_ZA
dc.type	Article	en_ZA