The evaluation of morphological characteristics of human spermatozoa according to stricter criteria

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dc.contributor.authorMenkveld R.
dc.contributor.authorStander F.S.H.
dc.contributor.authorKotze vW. T.J.
dc.contributor.authorKruger Van Zyl T.F.J.A.
dc.date.accessioned2011-05-15T16:16:35Z
dc.date.available2011-05-15T16:16:35Z
dc.date.issued1990
dc.description.abstractThe evaluation of the morphology of human spermatozoa varies widely between and sometimes even within laboratories. The purpose of this study was to determine whether the method that has been developed in our laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and to establish the within and between observer variations. The criteria used for a 'normal' spermatozoon are based on the appearance of spermatozoa found in the mucus of the upper endocervical canal. The results of the morphological evaluations of 26 samples by four observers were statistically analysed by various methods. The method of Barnett showed a high degree of relative accuracy between observers with error variances of between 2.89 and 19.67 as well as high Spearman rank correlation coefficients of between 0.8675 and 0.6537 (p < 0.0003). The Spearman correlation coefficient for 15 duplicate evaluations by one observer was 0.9650 (p < 0.0001) while the coefficients of variation for repeated evaluations of single samples were also within acceptable limits. Based on these results, the method described in this article allows comparable and reliable results between and within observers to be obtained. From this and other studies it can be concluded that the method also has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.
dc.description.versionArticle
dc.identifier.citationHuman Reproduction
dc.identifier.citation5
dc.identifier.citation5
dc.identifier.issn02681161
dc.identifier.urihttp://hdl.handle.net/10019.1/13850
dc.subjectarticle
dc.subjectcell culture
dc.subjectcytology
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectmeasurement
dc.subjectmethodology
dc.subjectmorphology
dc.subjectnormal human
dc.subjectnormal value
dc.subjectspermatozoon motility
dc.subjectuterine cervix mucus
dc.subjectComparative Study
dc.subjectCytodiagnosis
dc.subjectHuman
dc.subjectInfertility, Male
dc.subjectMale
dc.subjectQuality Control
dc.subjectSpecimen Handling
dc.subjectSpermatozoa
dc.subjectCricetinae
dc.titleThe evaluation of morphological characteristics of human spermatozoa according to stricter criteria
dc.typeArticle
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