Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s)
| dc.cibjournal | Cytokines | en_ZA |
| dc.contributor.author | Naidoo, Dhaneshree Bestinee | en_ZA |
| dc.contributor.author | Chuturgoon, Anil Amichund | en_ZA |
| dc.contributor.author | Phulukdaree, Alisa | en_ZA |
| dc.contributor.author | Guruprasad, Kanive Parashiva | en_ZA |
| dc.contributor.author | Satyamoorthy, Kapaettu | en_ZA |
| dc.contributor.author | Sewram, Vikash | en_ZA |
| dc.date.accessioned | 2018-04-16T05:52:08Z | |
| dc.date.available | 2018-04-16T05:52:08Z | |
| dc.date.issued | 2018-04-10 | |
| dc.date.updated | 2018-04-15T03:21:21Z | |
| dc.description | CITATION: Naidoo, D. B., et al. 2018. Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s). BMC Complementary and Alternative Medicine, 18:126, doi:10.1186/s12906-018-2192-y. | |
| dc.description | The original publication is available at https://bmccomplementalternmed.biomedcentral.com | |
| dc.description.abstract | Background: Cancer and inflammation are associated with cachexia. Withania somnifera (W. somnifera) possesses antioxidant and anti-inflammatory potential. We investigated the potential of an aqueous extract of the root of W. somnifera (WRE) to modulate cytokines, antioxidants and apoptosis in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s). Methods: Cytotoxcity of WRE was determined at 24 and 72 h (h). Oxidant scavenging activity of WRE was evaluated (2, 2-diphenyl-1 picrylhydrazyl assay). Glutathione (GSH) levels, caspase (− 8, − 9, − 3/7) activities and adenosine triphosphate (ATP) levels (Luminometry) were thereafter assayed. Tumour necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β and IL-10 levels were also assessed using enzyme-linked immunosorbant assay. Results: At 24 h, WRE (0.2–0.4 mg/ml) decreased PBMC viability between 20 and 25%, whereas it increased THP-1 viability between 15 and 23% (p < 0.001). At 72 h, WRE increased PBMC viability by 27–39% (0.05, 0.4 mg/ml WRE) whereas decreased THP-1 viability between 9 and 16% (0.05–0.4 mg/ml WRE) (p < 0.001). Oxidant scavenging activity was increased by WRE (0.05–0.4 mg/ml, p < 0.0001). PBMC TNF-α and IL-10 levels were decreased by 0.2–0.4 mg/ml WRE, whereas IL-1β levels were increased by 0.05–0.4 mg/ml WRE (p < 0.0001). In THP-1 cells, WRE (0.05–0.4 mg/ml) decreased TNF-α, IL-1β and IL-6 levels (p < 0.0001). At 24 h, GSH levels were decreased in PBMC’s, whilst increased in THP-1 cells by 0.2–0.4 mg/ml WRE (p < 0.0001). At 72 h, WRE (0.1–0.4 mg/ml) decreased GSH levels in both cell lines (p < 0.0001). At 24 h, WRE (0.2–0.4 mg/ml) increased PBMC caspase (-8, -3/7) activities whereas WRE (0.05, 0.1, 0.4 mg/ml) increased THP-1 caspase (-9, -3/7) activities (p < 0.0001). At 72 h, PBMC caspase (-8, -9, -3/7) activities were increased at 0.05–0.1 mg/ml WRE (p < 0.0001). In THP-1 cells, caspase (-8, -9, -3/7) activities and ATP levels were increased by 0.1–0.2 mg/ml WRE, whereas decreased by 0.05 and 0.4 mg/ml WRE (72 h, p < 0.0001). Conclusion: In PBMC’s and THP-1 cells,WRE proved to effectively modulate antioxidant activity, inflammatory cytokines and cell death. In THP-1 cells, WRE decreased pro-inflammatory cytokine levels, which may alleviate cancer cachexia and excessive leukaemic cell growth. | en |
| dc.description.uri | https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-018-2192-y | |
| dc.description.version | Publisher's version | |
| dc.format.extent | 11 pages ; illustrations | |
| dc.identifier.citation | Naidoo, D. B., et al. 2018. Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s). BMC Complementary and Alternative Medicine, 18:126, doi:10.1186/s12906-018-2192-y | |
| dc.identifier.issn | 1472-6882 (online) | |
| dc.identifier.other | doi:10.1186/s12906-018-2192-y | |
| dc.identifier.uri | http://hdl.handle.net/10019.1/103966 | |
| dc.language.iso | en_ZA | en_ZA |
| dc.publisher | BioMed Central | |
| dc.rights.holder | Authors retain copyright | |
| dc.subject | Leukemia -- Treatment | |
| dc.subject | Cancer -- Treatment | en_ZA |
| dc.subject | Withania somnifera | en_ZA |
| dc.subject | Cachexia | en_ZA |
| dc.subject | Cytokines | en_ZA |
| dc.title | Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s) | en_ZA |
| dc.type | Article | en_ZA |