Diguanylate cyclase null mutant reveals that C-Di-GMP pathway regulates the motility and adherence of the extremophile bacterium acidithiobacillus caldus
| dc.contributor.author | Castro, Matias | en_ZA |
| dc.contributor.author | Deane, Shelly M. | en_ZA |
| dc.contributor.author | Ruiz, Lina | en_ZA |
| dc.contributor.author | Rawlings, Douglas E. | en_ZA |
| dc.contributor.author | Guiliani, Nicolas | en_ZA |
| dc.date.accessioned | 2016-08-29T12:53:39Z | |
| dc.date.available | 2016-08-29T12:53:39Z | |
| dc.date.issued | 2015 | |
| dc.description | CITATION: Castro, M., et al. 2015. Diguanylate cyclase null mutant reveals that C-Di-GMP pathway regulates the motility and adherence of the extremophile bacterium acidithiobacillus caldus. PLoS ONE, 10(2):1-23, doi:10.1371/journal.pone.0116399. | |
| dc.description | The original publication is available at http://journals.plos.org/plosone | |
| dc.description.abstract | An understanding of biofilm formation is relevant to the design of biological strategies to improve the efficiency of the bioleaching process and to prevent environmental damages caused by acid mine/rock drainage. For this reason, our laboratory is focused on the characterization of the molecular mechanisms involved in biofilm formation in different biomining bacteria. In many bacteria, the intracellular levels of c-di-GMP molecules regulate the transition from the motile planktonic state to sessile community-based behaviors, such as biofilm development, through different kinds of effectors. Thus, we recently started a study of the c-di-GMP pathway in several biomining bacteria including Acidithiobacillus caldus. C-di-GMP molecules are synthesized by diguanylate cyclases (DGCs) and degraded by phosphodiesterases (PDEs). We previously reported the existence of intermediates involved in c-di-GMP pathway from different Acidithiobacillus species. Here, we report our work related to At. caldus ATCC 51756. We identified several putative-ORFs encoding DGC and PDE and effector proteins. By using total RNA extracted from At. caldus cells and RT-PCR, we demonstrated that these genes are expressed. We also demonstrated the presence of c-di-GMP by mass spectrometry and showed that genes for several of the DGC enzymes were functional by heterologous genetic complementation in Salmonella enterica serovar Typhimurium mutants. Moreover, we developed a DGC defective mutant strain (Δc1319) that strongly indicated that the c-di-GMP pathway regulates the swarming motility and adherence to sulfur surfaces by At. caldus. Together, our results revealed that At. caldus possesses a functional c-di-GMP pathway which could be significant for ores colonization during the bioleaching process. | en_ZA |
| dc.description.sponsorship | FONDECYT grants 1080441 and 1120295, MECESUP UCH0407, UCH-0604 | en_ZA |
| dc.description.sponsorship | VID-Universidad de Chile. NG: Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) (http://www.conicyt.cl/fondecyt/) | en_ZA |
| dc.description.sponsorship | MC:Programa de Mejoramiento de la Calidad y laEquidad de la Educación Superior (MECESUP) (http://www.mecesup.cl/), Universidad de Chile, UCH-0604 and VID-Universidad de Chile | en_ZA |
| dc.description.uri | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116399 | |
| dc.description.version | Publisher's version | |
| dc.format.extent | 23 pages | |
| dc.identifier.citation | Castro, M., et al. 2015. Diguanylate cyclase null mutant reveals that C-Di-GMP pathway regulates the motility and adherence of the extremophile bacterium acidithiobacillus caldus. PLoS ONE, 10(2):1-23, doi:10.1371/journal.pone.0116399 | |
| dc.identifier.issn | 1932-6203 (online) | |
| dc.identifier.other | doi:10.1371/journal.pone.0116399 | |
| dc.identifier.uri | http://hdl.handle.net/10019.1/99500 | |
| dc.language.iso | en_ZA | en_ZA |
| dc.publisher | Public Library of Science | |
| dc.rights.holder | Authors retain copyright | |
| dc.subject | Biofilm formation | en_ZA |
| dc.subject | Acidithiobacillus caldus | en_ZA |
| dc.subject | Extremophile bacterium acidithiobacillus caldus | en_ZA |
| dc.title | Diguanylate cyclase null mutant reveals that C-Di-GMP pathway regulates the motility and adherence of the extremophile bacterium acidithiobacillus caldus | en_ZA |
| dc.type | Article | en_ZA |