Die effek van steroied-terapie op die sitologiese en histopatologiese veranderinge tydens eksperimentele ekstrinsieke allergiese alveolitis (hipersensitiwiteitspneumonitis)

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dc.contributor.authorRossouw, D. J.en_ZA
dc.contributor.authorChase, C. C.en_ZA
dc.contributor.authorScheepers, J. C. E.en_ZA
dc.date.accessioned2011-03-18T14:57:45Z
dc.date.available2011-03-18T14:57:45Z
dc.date.issued1981
dc.descriptionThe original publication is available at http://www.samj.org.za
dc.descriptionCITATION: Rossouw, D. J., Chase, C. C. & Scheepers, J. C. E. 1981. Die effek van steroied-terapie op die sitologiese en histopatologiese veranderinge tydens eksperimentele ekstrinsieke allergiese alveolitis (hipersensitiwiteitspneumonitis). South African Medical Journal, 60:159-165.
dc.description.abstractAcute extrinsic allergic alveolitis was experimentally induced in rabbits using horseradish peroxidase (HRP) as antigen. Bronchoalveolar lavage was performed on the excised lungs and total and differential cellular yields determined, and correlated with the histopathological changes in the lungs as well as the total and differential white blood cell counts. After a single parenteral immunization with HRP without adjuvants, and weekly aerosol challenges with nebulized HRP solution for 3 consecutive weeks, a 3-fold increase in the total cell count as well as very pronounced rise in the percentage of lymphocytes was noticed. Histopathologically, the bronchi-associated lymphoid tissue dw-3 (BALT) became more prominent, an increase in the number of foreign body giant cells was noticed and a focal interstitial and intra-alveolar accumulation of lymphocytes, granulocytes and macrophages could be demonstrated, as well as a mild hyperplasia of type 2 alveolar epithelial cells. Intramuscular injections of methylprednisolone acetate (Depo-Medrol) every 72 hours induced a pronounced peripheral lymphopenia, thymic involution and an almost complete disappearance of the BALT in both the control and HRP-challenged rabbits. Similarly, a marked decrease in the total cell count and percentage of lymphocytes was noticed in the broncho-alveolar fluid of the animals with hypersensitivity pneumonitis. No signs of interstitial or intra-alveolar reactions were seen in the lungs of the experimental animals after 3 weeks of aerosol antigen challenge when treated with steroids. Collectively, these data suggest that the development of hypersensitivity pneumonitis was, at least in part, due to a cell-mediated immunological reaction in the lung. This animal model in which steroid suppression of experimental allergic alveolitis has been demonstrated, may be employed to elucidate the cellular pathogenesis of this disease process.
dc.description.versionPublisher’s version
dc.format.extent7 pages
dc.identifier.issn2078-5135 (online)
dc.identifier.issn0256-9574 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/7631
dc.language.isoaf
dc.publisherHealth & Medical Publishing Group
dc.rights.holderSouth African Medical Journal
dc.subjectHypersensitivity pneumonitisen_ZA
dc.titleDie effek van steroied-terapie op die sitologiese en histopatologiese veranderinge tydens eksperimentele ekstrinsieke allergiese alveolitis (hipersensitiwiteitspneumonitis)af_ZA
dc.title.alternativeThe effect of steroid therapy on the cytological and histopathological changes during experimental extrinsic allergic alveolitis (hypersensitivity pneumonitis)en_ZA
dc.typeArticle
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