The functional response of B cells to antigenic stimulation : a preliminary report of latent tuberculosis

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dc.contributor.authorDu Plessis, Willem J.en_ZA
dc.contributor.authorKleynhans, Leanieen_ZA
dc.contributor.authorDu Plessis, Nelitaen_ZA
dc.contributor.authorStanley, Kimen_ZA
dc.contributor.authorMalherbe, Stephanus T.en_ZA
dc.contributor.authorMaasdorp, Eliznaen_ZA
dc.contributor.authorRonacher, Katharinaen_ZA
dc.contributor.authorChegou, Novel N.en_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.date.accessioned2016-05-23T13:29:30Z
dc.date.available2016-05-23T13:29:30Z
dc.date.issued2016-04
dc.descriptionCITATION: Du Plessis, W. J., et al. 2016. The functional response of B cells to antigenic stimulation : a preliminary report of latent tuberculosis. PLoS ONE, 11(4): 1-16, doi: 10.1371/journal.pone.015271.en_ZA
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.descriptionThe original publication is available at http://journals.plos.org/plosoneen_ZA
dc.description.abstractMycobacterium tuberculosis (M.tb) remains a successful pathogen, causing tuberculosis disease numbers to constantly increase. Although great progress has been made in delineating the disease, the host-pathogen interaction is incompletely described. B cells have shown to function as both effectors and regulators of immunity via non-humoral methods in both innate and adaptive immune settings. Here we assessed specific B cell functional interaction following stimulation with a broad range of antigens within the LTBI milieu. Our results indicate that B cells readily produce pro- and anti-inflammatory cytokines (including IL-1β, IL-10, IL-17, IL-21 and TNF-α) in response to stimulation. TLR4 and TLR9 based stimulations achieved the greatest secreted cytokine-production response and BCG stimulation displayed a clear preference for inducing IL-1β production. We also show that the cytokines produced by B cells are implicated strongly in cell-mediated communication and that plasma (memory) B cells (CD19+CD27+CD138+) is the subset with the greatest contribution to cytokine production. Collectively our data provides insight into B cell responses, where they are implicated in and quantifies responses from specific B cell phenotypes. These findings warrant further functional B cell research with a focus on specific B cell phenotypes under conditions of active TB disease to further our knowledge about the contribution of various cell subsets which could have implications for future vaccine development or refined B cell orientated treatment in the health setting.en_ZA
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152710
dc.description.versionPublisher's versionen_ZA
dc.format.extent16 pages : illustrations (somer colour)en_ZA
dc.identifier.citationDu Plessis, W. J., et al. 2016. The functional response of B cells to antigenic stimulation : a preliminary report of latent tuberculosis. PLoS ONE, 11(4): 1-16, doi: 10.1371/journal.pone.015271
dc.identifier.issn1932-6203 (online)
dc.identifier.issn1932-6203 (print)
dc.identifier.otherdoi: 10.1371/journal.pone.015271
dc.identifier.urihttp://hdl.handle.net/10019.1/99003
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.subjectTuberculosisen_ZA
dc.subjectB cellsen_ZA
dc.subjectAntigenic stimulationen_ZA
dc.titleThe functional response of B cells to antigenic stimulation : a preliminary report of latent tuberculosisen_ZA
dc.typeArticleen_ZA
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