Effect of corticosterone on noradrenergic nuclei in the pons-medulla and [3H]NA release from terminals in hippocampal slices

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dc.contributor.authorDe Villiers A.S.
dc.contributor.authorRussell V.A.
dc.contributor.authorTaljaard J.J.F.
dc.contributor.authorDe Villiers A.S.
dc.contributor.authorRussell V.A.
dc.contributor.authorTaljaard J.J.F.
dc.date.accessioned2011-05-15T16:17:27Z
dc.date.accessioned2011-05-15T16:17:27Z
dc.date.available2011-05-15T16:17:27Z
dc.date.available2011-05-15T16:17:27Z
dc.date.issued1992
dc.date.issued1992
dc.description.abstractThe aim of the present study was to investigate possible membrane and genomic effects of corticosterone on the noradrenergic system of the rat brain. Corticosterone effects were studied in vivo by treating rats s.c. with 10 mg/kg corticosterone for 7 or 14 days. In the first two experiments corticosterone significantly decreased the noradrenaline (NA) and dopamine (DA) levels in the pons-medulla, an area which contains the A1-A7 noradrenergic cell groups, while the NA and DA levels in the dorsal hippocampus remained unchanged. In a third experiment where the locus coeruleus (LC) and the A1 and A2 nuclei (A1,A2) were analysed separately, NA levels were unchanged but total MHPG levels and the total MHPG/NA ratio were decreased in the A1,A2 area. Chronic corticosterone treatment (14 days) did not alter the α2-adrenoceptor-mediated modulation of [3H]NA release from dorsal hippocampal slices. Neither the spontaneous outflow nor the electrically stimulated release of [3H]NA from dorsal hippocampal slices of untreated rats was affected by exposure of the slices to corticosterone (10-7 M - 10-4 M) in the superfusion buffer. Thus, chronic corticosterone treatment of rats altered the noradrenergic system of the pons-medulla, but did not change the α2-adrenoceptor-mediated modulation of NA release in the dorsal hippocampus, a major terminal area of the LC neurons. Corticosterone also did not appear to have a direct membrane effect on the NA terminals in the dorsal hippocampus of the rat.
dc.description.abstractThe aim of the present study was to investigate possible membrane and genomic effects of corticosterone on the noradrenergic system of the rat brain. Corticosterone effects were studied in vivo by treating rats s.c. with 10 mg/kg corticosterone for 7 or 14 days. In the first two experiments corticosterone significantly decreased the noradrenaline (NA) and dopamine (DA) levels in the pons-medulla, an area which contains the A1-A7 noradrenergic cell groups, while the NA and DA levels in the dorsal hippocampus remained unchanged. In a third experiment where the locus coeruleus (LC) and the A1 and A2 nuclei (A1,A2) were analysed separately, NA levels were unchanged but total MHPG levels and the total MHPG/NA ratio were decreased in the A1,A2 area. Chronic corticosterone treatment (14 days) did not alter the α2-adrenoceptor-mediated modulation of [3H]NA release from dorsal hippocampal slices. Neither the spontaneous outflow nor the electrically stimulated release of [3H]NA from dorsal hippocampal slices of untreated rats was affected by exposure of the slices to corticosterone (10-7 M - 10-4 M) in the superfusion buffer. Thus, chronic corticosterone treatment of rats altered the noradrenergic system of the pons-medulla, but did not change the α2-adrenoceptor-mediated modulation of NA release in the dorsal hippocampus, a major terminal area of the LC neurons. Corticosterone also did not appear to have a direct membrane effect on the NA terminals in the dorsal hippocampus of the rat.
dc.description.versionArticle
dc.description.versionArticle
dc.identifier.citationNeurochemical Research
dc.identifier.citation17
dc.identifier.citation3
dc.identifier.citationNeurochemical Research
dc.identifier.citation17
dc.identifier.citation3
dc.identifier.issn03643190
dc.identifier.issn03643190
dc.identifier.urihttp://hdl.handle.net/10019.1/14223
dc.identifier.urihttp://hdl.handle.net/10019.1/14223
dc.subjectalpha 2 adrenergic receptor; corticosterone; dopamine; noradrenalin; serotonin; animal tissue; article; brain stem; controlled study; corticosterone release; dopamine release; drug blood level; drug effect; drug receptor binding; drug tissue level; electrostimulation; hippocampus; locus ceruleus; male; nonhuman; noradrenalin release; noradrenergic system; priority journal; rat; subcutaneous drug administration; Animal; Biogenic Monoamines; Corticosterone; Hippocampus; In Vitro; Male; Medulla Oblongata; Methoxyhydroxyphenylglycol; Nerve Endings; Norepinephrine; Perfusion; Pons; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't; Tritium
dc.subjectalpha 2 adrenergic receptor
dc.subjectcorticosterone
dc.subjectdopamine
dc.subjectnoradrenalin
dc.subjectserotonin
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectbrain stem
dc.subjectcontrolled study
dc.subjectcorticosterone release
dc.subjectdopamine release
dc.subjectdrug blood level
dc.subjectdrug effect
dc.subjectdrug receptor binding
dc.subjectdrug tissue level
dc.subjectelectrostimulation
dc.subjecthippocampus
dc.subjectlocus ceruleus
dc.subjectmale
dc.subjectnonhuman
dc.subjectnoradrenalin release
dc.subjectnoradrenergic system
dc.subjectpriority journal
dc.subjectrat
dc.subjectsubcutaneous drug administration
dc.subjectAnimal
dc.subjectBiogenic Monoamines
dc.subjectCorticosterone
dc.subjectHippocampus
dc.subjectIn Vitro
dc.subjectMale
dc.subjectMedulla Oblongata
dc.subjectMethoxyhydroxyphenylglycol
dc.subjectNerve Endings
dc.subjectNorepinephrine
dc.subjectPerfusion
dc.subjectPons
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.subjectSupport, Non-U.S. Gov't
dc.subjectTritium
dc.titleEffect of corticosterone on noradrenergic nuclei in the pons-medulla and [3H]NA release from terminals in hippocampal slices
dc.titleEffect of corticosterone on noradrenergic nuclei in the pons-medulla and [3H]NA release from terminals in hippocampal slices
dc.typeArticle
dc.typeArticle
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