Clonal expansion of colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africa
| dc.contributor.author | Snyman, Yolandi | en_ZA |
| dc.contributor.author | Whitelaw, Andrew Christopher | en_ZA |
| dc.contributor.author | Reuter, Sandra | en_ZA |
| dc.contributor.author | Dramowski, Angela | en_ZA |
| dc.contributor.author | Maloba, Motlatji Reratilwe Bonnie | en_ZA |
| dc.contributor.author | Newton-Foot, Mae | en_ZA |
| dc.date.accessioned | 2022-09-14T08:59:43Z | en_ZA |
| dc.date.available | 2022-09-14T08:59:43Z | en_ZA |
| dc.date.issued | 2020-02 | en_ZA |
| dc.description | CITATION: Snyman, Y. et al. 2020. Clonal expansion of colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africa. International journal of infectious diseases. International Society for Infectious Diseases, 91:94–100. doi:10.1016/j.ijid.2019.11.021 | en_ZA |
| dc.description | The original publication is available at https://www.journals.elsevier.com/international-journal-of-infectious-diseases | en_ZA |
| dc.description.abstract | Objectives: To describe colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africa. Methods: A. baumannii isolates identified on Vitek 2 Advanced Expert System were collected from Tygerberg Hospital referral laboratory between 2016 and 2017. Colistin resistance was confirmed using broth microdilution and SensiTest. mcr-1-5 were detected using PCR and strain typing was performed by rep-PCR. Whole genome sequencing (WGS) was performed on a subset of isolates to identify chromosomal colistin resistance mechanisms and strain diversity using multilocus sequence typing (MLST) and pairwise single nucleotide polymorphism analyses. Results: Twenty-six colistin-resistant and six colistin-susceptible A. baumannii were collected separately based on Vitek susceptibility; 20/26 (77%) were confirmed colistin-resistant by broth microdilution. Four colistin-resistant isolates were isolated in 2016 and 16 in 2017, from five healthcare facilities. Thirteen colistin-resistant isolates and eight colistin-susceptible isolates were identical by rep-PCR and MLST (ST1), all from patients admitted to a tertiary hospital during 2017. The remaining colistin-resistant isolates were unrelated. Conclusions: An increase in colistin-resistant A. baumannii isolates from a tertiary hospital in 2017 appears to be clonal expansion of an emerging colistin-resistant strain. This strain was not detected in 2016 or from other hospitals. Identical colistin-susceptible isolates were also isolated, suggesting relatively recent acquisition of colistin resistance. | en_ZA |
| dc.description.uri | https://www.clinicalkey.com/#!/content/playContent/1-s2.0-S1201971219304606?returnurl=https:%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1201971219304606%3Fshowall%3Dtrue&referrer=https:%2F%2Fpubmed.ncbi.nlm.nih.gov%2F | en_ZA |
| dc.description.version | Publishers version | en_ZA |
| dc.format.extent | 7 pages | en_ZA |
| dc.identifier.citation | Snyman, Y. et al. 2020. Clonal expansion of colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africa. International journal of infectious diseases. International Society for Infectious Diseases, 91:94–100. doi:10.1016/j.ijid.2019.11.021 | en_ZA |
| dc.identifier.issn | 1878-3511 (online) | en_ZA |
| dc.identifier.issn | 1201-9712 (print) | en_ZA |
| dc.identifier.other | doi:10.1016/j.ijid.2019.11.021 | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/10019.1/125725 | en_ZA |
| dc.language.iso | en_ZA | en_ZA |
| dc.publisher | Elsevier | en_ZA |
| dc.rights.holder | Authors retain copyright | en_ZA |
| dc.subject | Acinetobacter infections | en_ZA |
| dc.subject | Clonal expansion | en_ZA |
| dc.subject | Colistin resistance | en_ZA |
| dc.subject | Nosocomial infections -- South Africa | en_ZA |
| dc.title | Clonal expansion of colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africa | en_ZA |
| dc.type | Article | en_ZA |