Migration of bacteriocins across gastrointestinal epithelial and vascular endothelial cells, as determined using in vitro simulations

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dc.contributor.authorDreyer, Leaneen_ZA
dc.contributor.authorSmith, Carineen_ZA
dc.contributor.authorDeane, Shelly M.en_ZA
dc.contributor.authorDicks, Leon Milner Theodore, 1961-en_ZA
dc.contributor.authorVan Staden, Anton D.en_ZA
dc.date.accessioned2019-08-21T13:57:55Z
dc.date.available2019-08-21T13:57:55Z
dc.date.issued2019-08-07
dc.descriptionCITATION: Dreyer, L., et al. 2019. Migration of bacteriocins across gastrointestinal epithelial and vascular endothelial cells, as determined using in vitro simulations. Scientific Reports, 9:11481, doi:10.1038/s41598-019-47843-9.en_ZA
dc.descriptionThe original publication is available at https://www.nature.comen_ZA
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Funden_ZA
dc.description.abstractLittle is known about the migration of bacteriocins across human cells. In this study, we report on migration of three bacteriocins nisin, plantaricin 423 and bacST4SA across colonic adenocarcinoma (Caco-2) cells and human umbilical vein endothelial cells (HUVECs). Bacteriocins were fluorescently labelled while still maintaining antimicrobial activity. Migration of fluorescently labelled bacteriocins across monolayers was assessed in vitro using transmigration well inserts. After 3 h, 75% of nisin, 85% of plantaricin 423 and 82% of bacST4SA migrated across the Caco-2 cell monolayer. Over the same time span, 88% nisin, 93% plantaricin 423 and 91% bacST4SA migrated across the HUVEC monolayer. The viability of both cell types remained unchanged when exposed to 50 µM of nisin, plantaricin 423 or bacST4SA. The effect of human plasma on bacteriocin activity was also assessed. Activity loss was dependent on bacteriocin type and concentration, with the class-IIa bacteriocins retaining more activity compared to nisin. This is the first report of bacteriocins migrating across simulated gastrointestinal- and vascular-barriers. This study provides some of the first evidence that bacteriocins are capable of crossing the gut-blood-barrier. However, in vivo studies need to be performed to confirm these findings and expand on the role of bacteriocin migration across cell barriersen_ZA
dc.description.urihttps://www.nature.com/articles/s41598-019-47843-9
dc.description.versionPublisher's versionen_ZA
dc.format.extent11 pages : illustrationsen_ZA
dc.identifier.citationDreyer, L., et al. 2019. Migration of bacteriocins across gastrointestinal epithelial and vascular endothelial cells, as determined using in vitro simulations. Scientific Reports, 9:11481, doi:10.1038/s41598-019-47843-9en_ZA
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/s41598-019-47843-9
dc.identifier.urihttp://hdl.handle.net/10019.1/106371
dc.language.isoen_ZAen_ZA
dc.publisherNature Research (part of Springer Nature)en_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectBacteriocinsen_ZA
dc.subjectGastrointestinal epithelial cellsen_ZA
dc.subjectVascular endothelial cellsen_ZA
dc.subjectBacteriocins -- Effect of human plasma onen_ZA
dc.subjectBacteriocins -- Migrationen_ZA
dc.titleMigration of bacteriocins across gastrointestinal epithelial and vascular endothelial cells, as determined using in vitro simulationsen_ZA
dc.typeArticleen_ZA
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