Complement component C1q as serum biomarker to detect active tuberculosis

Simple item page
dc.contributor.authorLubbers, Rosalieen_ZA
dc.contributor.authorSutherland, Jayne S.en_ZA
dc.contributor.authorGoletti, Deliaen_ZA
dc.contributor.authorDe Paus, Roelof A.en_ZA
dc.contributor.authorVan Moorsel, Coline H. M.en_ZA
dc.contributor.authorVeltkamp, Marcelen_ZA
dc.contributor.authorVestjens, Stefan M. T.en_ZA
dc.contributor.authorBos, Willem J. W.en_ZA
dc.contributor.authorPetrone, Lindaen_ZA
dc.contributor.authorDel Nonno, Francaen_ZA
dc.contributor.authorBajema, Ingeborg M.en_ZA
dc.contributor.authorDijkman, Karinen_ZA
dc.contributor.authorVerreck, Frank A. W.en_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorGelderman, Kyra A.en_ZA
dc.contributor.authorGroeneveld, Geert H.en_ZA
dc.contributor.authorGeluk, Annemiekeen_ZA
dc.contributor.authorOttenhoff, Tom H. M.en_ZA
dc.contributor.authorJoosten, Simone A.en_ZA
dc.contributor.authorTrouw, Leendert A.en_ZA
dc.date.accessioned2019-09-25T12:15:33Z
dc.date.available2019-09-25T12:15:33Z
dc.date.issued2018
dc.descriptionCITATION: Lubbers, R., et al. 2018. Complement component C1q as serum biomarker to detect active tuberculosis. Frontiers in Immunology, 9:2427, doi:10.3389/fimmu.2018.02427.
dc.descriptionThe original publication is available at https://www.frontiersin.org
dc.description.abstractBackground: Tuberculosis (TB) remains a major threat to global health. Currently, diagnosis of active TB is hampered by the lack of specific biomarkers that discriminate active TB disease from other (lung) diseases or latent TB infection (LTBI). Integrated human gene expression results have shown that genes encoding complement components, in particular different C1q chains, were expressed at higher levels in active TB compared to LTBI. Methods: C1q protein levels were determined using ELISA in sera from patients, from geographically distinct populations, with active TB, LTBI as well as disease controls. Results: Serum levels of C1q were increased in active TB compared to LTBI in four independent cohorts with an AUC of 0.77 [0.70; 0.83]. After 6 months of TB treatment, levels of C1q were similar to those of endemic controls, indicating an association with disease rather than individual genetic predisposition. Importantly, C1q levels in sera of TB patients were significantly higher as compared to patients with sarcoidosis or pneumonia, clinically important differential diagnoses. Moreover, exposure to other mycobacteria, such as Mycobacterium leprae (leprosy patients) or BCG (vaccinees) did not result in elevated levels of serum C1q. In agreement with the human data, in non-human primates challenged with Mycobacterium tuberculosis, increased serum C1q levels were detected in animals that developed progressive disease, not in those that controlled the infection. Conclusions: In summary, C1q levels are elevated in patients with active TB compared to LTBI in four independent cohorts. Furthermore, C1q levels from patients with TB were also elevated compared to patients with sarcoidosis, leprosy and pneumonia. Additionally, also in NHP we observed increased C1q levels in animals with active progressive TB, both in serum and in broncho-alveolar lavage. Therefore, we propose that the addition of C1q to current biomarker panels may provide added value in the diagnosis of active TB.en_ZA
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fimmu.2018.02427/full
dc.description.versionPublisher's version
dc.format.extent12 pagesen_ZA
dc.identifier.citationLubbers, R., et al. 2018. Complement component C1q as serum biomarker to detect active tuberculosis. Frontiers in Immunology, 9:2427, doi:10.3389/fimmu.2018.02427
dc.identifier.issn1664-3224 (online)
dc.identifier.otherdoi:10.3389/fimmu.2018.02427
dc.identifier.urihttp://hdl.handle.net/10019.1/106520
dc.language.isoen_ZAen_ZA
dc.publisherFrontiers Mediaen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectTuberculosis -- Diagnosisen_ZA
dc.subjectMycobacterium lepraeen_ZA
dc.subjectMycobacteriumen_ZA
dc.subjectBiochemical markersen_ZA
dc.titleComplement component C1q as serum biomarker to detect active tuberculosisen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
lubbers_complement_2018.pdf
Size:
2.1 MB
Format:
Adobe Portable Document Format
Description:
Download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Molecular Biology and Human Genetics)