Defective sperm decondensation: A cause for fertilization failure

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dc.contributor.authorEsterhuizen A.D.
dc.contributor.authorFranken D.R.
dc.contributor.authorBecker P.J.
dc.contributor.authorLourens J.G.H.
dc.contributor.authorMuller I.I.
dc.contributor.authorVan Rooyen L.H.
dc.date.accessioned2011-05-15T16:15:25Z
dc.date.available2011-05-15T16:15:25Z
dc.date.issued2002
dc.description.abstractThe study aimed to evaluate the role of chromatin packaging (CMA3 staining), sperm morphology during sperm-zona binding, sperm decondensation and the presence of polar bodies in oocytes that failed in vitro fertilization (IVF). The percentage CMA3 staining categorized the data into three groups, <44%, n=10; ≥44-59%, n=10; and ≥60%, n=29. Morphology groups were ≤4% (n=11); >4-14% (n=19); and >14% (n=19). One hundred and seventy-two oocytes that failed IVF were evaluated for sperm-zona binding, ooplasma penetration and sperm decondensation. Odds ratio analyses indicated that being in the ≥60% CMA3 staining group resulted in a 15.6 fold increase in the risk of decondensation failure, relative to CMA3 staining of <44%. For morphology, there was a 2.17 fold decrease in the risk of fertilization failure in the morphology group with >4-14% normal cells, while it increased 2.45 fold for the morphology group with ≤4% normal cells. Using CMA3 fluorescence to discriminate, 51% of the oocytes in the group with elevated CMA3 fluorescence had no sperm in the ooplasma compared to 32% and 16% penetration failure in the CMA3 staining groups ≥44-59% and <44%, respectively. Sperm chromatin packaging quality and sperm morphology assessments are useful clinical indicators of human fertilization failure. Immunofluorescence techniques could be used to provide a clear diagnosis of failed fertilization.
dc.description.versionArticle
dc.identifier.citationAndrologia
dc.identifier.citation34
dc.identifier.citation1
dc.identifier.issn03034569
dc.identifier.other10.1046/j.0303-4569.2001.00423.x
dc.identifier.urihttp://hdl.handle.net/10019.1/13331
dc.subjectadult
dc.subjectarticle
dc.subjectbinding affinity
dc.subjectcell structure
dc.subjectchromatin
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectfertilization in vitro
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunofluorescence
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectoocyte
dc.subjectovulation induction
dc.subjectspermatozoon
dc.subjectspermatozoon abnormality
dc.subjectspermatozoon penetration
dc.subjectstaining
dc.subjectzona pellucida
dc.subjectChromatin
dc.subjectFemale
dc.subjectFertilization in Vitro
dc.subjectFluorescent Antibody Technique
dc.subjectHumans
dc.subjectInfertility, Male
dc.subjectMale
dc.subjectMicroscopy, Fluorescence
dc.subjectOdds Ratio
dc.subjectSperm Injections, Intracytoplasmic
dc.subjectSpermatozoa
dc.subjectStaining and Labeling
dc.subjectTreatment Failure
dc.titleDefective sperm decondensation: A cause for fertilization failure
dc.typeArticle
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