Aspalathin-enriched green rooibos extract reduces hepatic insulin resistance by modulating PI3K/AKT and AMPK pathways
dc.contributor.author | Mazibuko-Mbeje, Sithandiwe E. | en_ZA |
dc.contributor.author | Dludla, Phiwayinkosi V. | en_ZA |
dc.contributor.author | Roux, Candice | en_ZA |
dc.contributor.author | Johnson, Rabia | en_ZA |
dc.contributor.author | Ghoor, Samira | en_ZA |
dc.contributor.author | Joubert, Elizabeth | en_ZA |
dc.contributor.author | Louw, Johan | en_ZA |
dc.date.accessioned | 2021-09-30T12:57:41Z | |
dc.date.available | 2021-09-30T12:57:41Z | |
dc.date.issued | 2019-02-01 | |
dc.description | CITATION: Mazibuko-Mbeje, S. E. et al. 2019. Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways. International Journal of Molecular Sciences, 20(3). doi:10.3390/ijms20030633 | |
dc.description | The original publication is available at https://www.mdpi.com/journal/ijms | |
dc.description.abstract | We previously demonstrated that an aspalathin-enriched green rooibos extract (GRE) reversed palmitate-induced insulin resistance in C2C12 skeletal muscle and 3T3-L1 fat cells by modulating key effectors of insulin signalling such as phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B (PI3K/AKT) and AMP-activated protein kinase (AMPK). However, the effect of GRE on hepatic insulin resistance is unknown. The effects of GRE on lipid-induced hepatic insulin resistance using palmitate-exposed C3A liver cells and obese insulin resistant (OBIR) rats were explored. GRE attenuated the palmitate-induced impairment of glucose and lipid metabolism in treated C3A cells and improved insulin sensitivity in OBIR rats. Mechanistically, GRE treatment significantly increased PI3K/AKT and AMPK phosphorylation while concurrently enhancing glucose transporter 2 expression. These findings were further supported by marked stimulation of genes involved in glucose metabolism, such as insulin receptor (Insr) and insulin receptor substrate 1 and 2 (Irs1 and Irs2), as well as those involved in lipid metabolism, including Forkhead box protein O1 (FOXO1) and carnitine palmitoyl transferase 1 (CPT1) following GRE treatment. GRE showed a strong potential to ameliorate hepatic insulin resistance by improving insulin sensitivity through the regulation of PI3K/AKT, FOXO1 and AMPK-mediated pathways. | en_ZA |
dc.description.sponsorship | National Research Foundation | |
dc.description.uri | https://www.mdpi.com/1422-0067/20/3/633/htm | |
dc.description.version | Publisher’s version | |
dc.format.extent | 16 pages | |
dc.identifier.citation | Mazibuko-Mbeje, S. E. et al. 2019. Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways. International Journal of Molecular Sciences, 20(3). doi:10.3390/ijms20030633 | |
dc.identifier.issn | 1422-0067 (online) | |
dc.identifier.issn | 1661-6596 (print) | |
dc.identifier.other | doi:10.3390/ijms20030633 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/123108 | |
dc.language.iso | en_ZA | en_ZA |
dc.publisher | MDPI | |
dc.rights.holder | Authors retain rights | |
dc.subject | Metabolic syndrome -- Alternative treatment | en_ZA |
dc.subject | Obesity -- Diseases -- Diet therapy | en_ZA |
dc.subject | Insulin resistance -- Diet therapy | en_ZA |
dc.subject | Diabetes mellitus | en_ZA |
dc.subject | Green rooibos extract -- Therapeutic use | en_ZA |
dc.subject | Therapeutic target | en_ZA |
dc.subject | Lipids -- Metabolism -- Disorders | en_ZA |
dc.subject | PI3K | en_ZA |
dc.subject | AKT | en_ZA |
dc.subject | AMPK | en_ZA |
dc.title | Aspalathin-enriched green rooibos extract reduces hepatic insulin resistance by modulating PI3K/AKT and AMPK pathways | en_ZA |
dc.type | Article | en_ZA |