Aspalathin-enriched green rooibos extract reduces hepatic insulin resistance by modulating PI3K/AKT and AMPK pathways

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dc.contributor.authorMazibuko-Mbeje, Sithandiwe E.en_ZA
dc.contributor.authorDludla, Phiwayinkosi V.en_ZA
dc.contributor.authorRoux, Candiceen_ZA
dc.contributor.authorJohnson, Rabiaen_ZA
dc.contributor.authorGhoor, Samiraen_ZA
dc.contributor.authorJoubert, Elizabethen_ZA
dc.contributor.authorLouw, Johanen_ZA
dc.date.accessioned2021-09-30T12:57:41Z
dc.date.available2021-09-30T12:57:41Z
dc.date.issued2019-02-01
dc.descriptionCITATION: Mazibuko-Mbeje, S. E. et al. 2019. Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways. International Journal of Molecular Sciences, 20(3). doi:10.3390/ijms20030633
dc.descriptionThe original publication is available at https://www.mdpi.com/journal/ijms
dc.description.abstractWe previously demonstrated that an aspalathin-enriched green rooibos extract (GRE) reversed palmitate-induced insulin resistance in C2C12 skeletal muscle and 3T3-L1 fat cells by modulating key effectors of insulin signalling such as phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B (PI3K/AKT) and AMP-activated protein kinase (AMPK). However, the effect of GRE on hepatic insulin resistance is unknown. The effects of GRE on lipid-induced hepatic insulin resistance using palmitate-exposed C3A liver cells and obese insulin resistant (OBIR) rats were explored. GRE attenuated the palmitate-induced impairment of glucose and lipid metabolism in treated C3A cells and improved insulin sensitivity in OBIR rats. Mechanistically, GRE treatment significantly increased PI3K/AKT and AMPK phosphorylation while concurrently enhancing glucose transporter 2 expression. These findings were further supported by marked stimulation of genes involved in glucose metabolism, such as insulin receptor (Insr) and insulin receptor substrate 1 and 2 (Irs1 and Irs2), as well as those involved in lipid metabolism, including Forkhead box protein O1 (FOXO1) and carnitine palmitoyl transferase 1 (CPT1) following GRE treatment. GRE showed a strong potential to ameliorate hepatic insulin resistance by improving insulin sensitivity through the regulation of PI3K/AKT, FOXO1 and AMPK-mediated pathways.en_ZA
dc.description.sponsorshipNational Research Foundation
dc.description.urihttps://www.mdpi.com/1422-0067/20/3/633/htm
dc.description.versionPublisher’s version
dc.format.extent16 pages
dc.identifier.citationMazibuko-Mbeje, S. E. et al. 2019. Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways. International Journal of Molecular Sciences, 20(3). doi:10.3390/ijms20030633
dc.identifier.issn1422-0067 (online)
dc.identifier.issn1661-6596 (print)
dc.identifier.otherdoi:10.3390/ijms20030633
dc.identifier.urihttp://hdl.handle.net/10019.1/123108
dc.language.isoen_ZAen_ZA
dc.publisherMDPI
dc.rights.holderAuthors retain rights
dc.subjectMetabolic syndrome -- Alternative treatmenten_ZA
dc.subjectObesity -- Diseases -- Diet therapyen_ZA
dc.subjectInsulin resistance -- Diet therapyen_ZA
dc.subjectDiabetes mellitusen_ZA
dc.subjectGreen rooibos extract -- Therapeutic useen_ZA
dc.subjectTherapeutic targeten_ZA
dc.subjectLipids -- Metabolism -- Disordersen_ZA
dc.subjectPI3Ken_ZA
dc.subjectAKTen_ZA
dc.subjectAMPKen_ZA
dc.titleAspalathin-enriched green rooibos extract reduces hepatic insulin resistance by modulating PI3K/AKT and AMPK pathwaysen_ZA
dc.typeArticleen_ZA
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