Decreased expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4+ T cells and peripheral blood from tuberculosis patients

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dc.contributor.authorKleinsteuber, Katjaen_ZA
dc.contributor.authorHeesch, Kerrinen_ZA
dc.contributor.authorSchattling, Stefanieen_ZA
dc.contributor.authorKohns, Malteen_ZA
dc.contributor.authorSander-Julch, Claudiaen_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorHesseling, Annekeen_ZA
dc.contributor.authorMayatepek, Ertanen_ZA
dc.contributor.authorFleischer, Bernharden_ZA
dc.contributor.authorMarx, Florian M.en_ZA
dc.contributor.authorJacobsen, Marcen_ZA
dc.date.accessioned2014-07-07T12:33:22Z
dc.date.available2014-07-07T12:33:22Z
dc.date.issued2013-04-16
dc.descriptionCITATION: Kleinsteuber, K. et al. 2013. Decreased expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4+ T cells and peripheral blood from tuberculosis patients. PLoS ONE, 8(4): e61609, doi:10.1371/journal.pone.0061609.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractThe vast majority of Mycobacterium tuberculosis (M. tuberculosis) infected individuals are protected from developing tuberculosis and T cells are centrally involved in this process. MicroRNAs (miRNA) regulate T-cell functions and are biomarker candidates of disease susceptibility and treatment efficacy in M. tuberculosis infection. We determined the expression profile of 29 selected miRNAs in CD4+ T cells from tuberculosis patients and contacts with latent M. tuberculosis infection (LTBI). These analyses showed lower expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4+ T cells from tuberculosis patients. Whole blood miRNA candidate analyses verified decreased expression of miR-26a, miR-29a, and miR-142-3p in children with tuberculosis as compared to healthy children with LTBI. Despite marked variances between individual donor samples, trends of increased miRNA candidate expression during treatment and recovery were observed. Functional in vitro analysis identified increased miR-21 and decreased miR-26a expression after re-stimulation of T cells. In vitro polarized Interleukin-17 positive T-cell clones showed activation-dependent miR-29a up-regulation. In order to characterize the role of miR-29a (a described suppressor of Interferon-γ in tuberculosis), we analyzed M. tuberculosis specific Interferon-γ expressing T cells in children with tuberculosis and healthy contacts but detected no correlation between miR-29a and Interferon-γ expression. Suppression of miR-29a in primary human T cells by antagomirs indicated no effect on Interferon-γ expression after in vitro activation. Finally, classification of miRNA targets revealed only a moderate overlap between the candidates. This may reflect differential roles of miR-21, miR-26a, miR-29a, and miR-142-3p in T-cell immunity against M. tuberculosis infection and disease.
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0061609
dc.description.versionPublisher's version
dc.format.extent10 pages
dc.identifier.citationKleinsteuber, K. et al. 2013. Decreased expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4+ T cells and peripheral blood from tuberculosis patients. PLoS ONE, 8(4): e61609, doi:10.1371/journal.pone.0061609.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0061609
dc.identifier.urihttp://hdl.handle.net/10019.1/94809
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectTuberculosisen_ZA
dc.subjectRNAen_ZA
dc.titleDecreased expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4+ T cells and peripheral blood from tuberculosis patientsen_ZA
dc.typeArticle
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