Hypoxia and the heart : the role of nitric oxide in cardiac myocytes and endothelial cells

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dc.contributor.advisorLochner, Amanda
dc.contributor.advisorMoolman, Johan
dc.contributor.authorStrijdom, Hans
dc.contributor.otherUniversity of Stellenbosch. Faculty of Health Sciences. Dept. of Biomedical Sciences. Medical Physiology.
dc.date.accessioned2007-11-13T06:30:08Zen_ZA
dc.date.accessioned2010-06-01T08:14:29Z
dc.date.available2007-11-13T06:30:08Zen_ZA
dc.date.available2010-06-01T08:14:29Z
dc.date.issued2007-03
dc.descriptionThesis (PhD (Biomedical Sciences. Medical Physiology))--University of Stellenbosch, 2007.
dc.description.abstractNitric oxide (NO) is a major signaling molecule in the heart with various biological effects. The putative role of NO as a cardioprotective agent against ischaemiareperfusion injury and in ischaemic preconditioning (IP) has made it one of the fastest growing fields in basic cardiovascular research. However, NO may also be associated with harmful effects, especially when released in excessive amounts. Little is known about the relative contributions to NO-production by the cardiac microvascular endothelial cells (CMECs) and the adjacent cardiomyocytes. Furthermore, the respective roles of endothelial NOS (eNOS) and inducible NOS (iNOS) are not well characterized in these cell types, particularly in hypoxia. In order to gain a better understanding of the role of NO in the hypoxic/ischaemic heart, the aims of this study were to: (1) develop an isolated cardiomyocyte model in which hypoxia and early IP can be induced and the role of NO assessed; (2) measure NOproduction in cardiomyocytes and CMECs under baseline and hypoxic conditions; and (3) evaluate the expression, regulation and activation of eNOS and iNOS in cardiomyocytes and CMECs (baseline and hypoxia) and establish the relationship with NO-production under these conditions. Cardiomyocytes isolated from adult rat hearts and commercially purchased rat CMECs were used as cell models.en
dc.format.extent4058212 bytesen_ZA
dc.format.mimetypeapplication/pdfen_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/1184
dc.language.isoen
dc.publisherStellenbosch : University of Stellenbosch
dc.rights.holderUniversity of Stellenbosch
dc.subjectAnoxemiaen
dc.subjectNitric oxideen
dc.subjectMyocardiumen
dc.subjectCardiovascular system -- Diseasesen
dc.subjectTheses -- Medical physiologyen
dc.subjectDissertations -- Medical physiologyen
dc.subjectMedicineen
dc.titleHypoxia and the heart : the role of nitric oxide in cardiac myocytes and endothelial cellsen
dc.typeThesis
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