High resolution imaging study of interactions between the 37 kDa/67 kDa Laminin receptor and APP, beta-secretase and gamma-secretase in Alzheimer’s Disease

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dc.contributor.authorJovanovic, Katarinaen_ZA
dc.contributor.authorLoos, Benen_ZA
dc.contributor.authorDa Costa Dias, Biancaen_ZA
dc.contributor.authorPenny, Clementen_ZA
dc.contributor.authorWeiss, Stefan F. T.en_ZA
dc.date.accessioned2016-03-01T06:28:32Z
dc.date.available2016-03-01T06:28:32Z
dc.date.issued2014-06-27
dc.descriptionCITATION: Jovanovic, K. et al. 2014. High resolution imaging study of interactions between the 37 kDa/67 kDa Laminin receptor and APP, beta-secretase and gamma-secretase in Alzheimer’s Disease. PLoS ONE, 9(6):e100373, doi:10.1371/journal.pone.0100373.
dc.descriptionThe original publication is available at http://journals.plos.org/plosoneen_ZA
dc.description.abstractAlzheimer's disease (AD) is the most prevalent form of dementia affecting the elderly. Neurodegeneration is caused by the amyloid beta (Aβ) peptide which is generated from the sequential proteolytic cleavage of the Amyloid Precursor Protein (APP) by the β– and γ- secretases. Previous reports revealed that the 37 kDa/67 kDa laminin receptor (LRP/LR) is involved in APP processing, however, the exact mechanism by which this occurs remains largely unclear. This study sought to assess whether LRP/LR interacted with APP, β- or γ-secretase. Detailed confocal microscopy revealed that LRP/LR showed a strong co-localisation with APP, β- and γ-secretase, respectively, at various sub-cellular locations. Superresolution Structured Illumination Microscopy (SR-SIM) showed that interactions were unlikely between LRP/LR and APP and β-secretase, respectively, while there was strong co-localisation between LRP/LR and γ-secretase at this 80 nm resolution. FRET was further employed to assess the possibility of protein-protein interactions and only an interaction between LRP/LR and γ-secretase was found. FLAG co-immunoprecipitation confirmed these findings as LRP/LR co-immunoprecipitated with γ-secretase, but failed to do so with APP. These findings indicate that LRP/LR exerts its influence on Aβ shedding via a direct interaction with the γ-secretase and possibly an indirect interaction with the β-secretase.en_ZA
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0100373
dc.description.versionPublisher's versionen_ZA
dc.format.extent10 pagesen_ZA
dc.identifier.citationJovanovic, K. et al. 2014. High resolution imaging study of interactions between the 37 kDa/67 kDa Laminin receptor and APP, beta-secretase and gamma-secretase in Alzheimer’s Disease. PLoS ONE, 9(6):e100373, doi:10.1371/journal.pone.0100373.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0100373
dc.identifier.urihttp://hdl.handle.net/10019.1/98236
dc.language.isoen_ZAen_ZA
dc.publisherPLoS
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectAlzheimer's disease -- Etiologyen_ZA
dc.titleHigh resolution imaging study of interactions between the 37 kDa/67 kDa Laminin receptor and APP, beta-secretase and gamma-secretase in Alzheimer’s Diseaseen_ZA
dc.typeArticleen_ZA
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