Differential expression of host biomarkers in saliva and serum samples from individuals with suspected pulmonary tuberculosis

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dc.contributor.authorPhalane, Khutso G.en_ZA
dc.contributor.authorKriel, Magdalenaen_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.contributor.authorMenezes, Angelaen_ZA
dc.contributor.authorStanley, Kimen_ZA
dc.contributor.authorVan der Spuy, Gian D.en_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorChegou, Novel N.en_ZA
dc.date.accessioned2014-02-11T09:13:33Z
dc.date.available2014-02-11T09:13:33Z
dc.date.issued2013-09
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.descriptionThe original publication is available at http://www.hindawi.com/journals/mi/en_ZA
dc.descriptionCITATION: Phalane, K. G. et al. 2013. Differential expression of host biomarkers in saliva and serum samples from individuals with suspected pulmonary tuberculosis. Mediators of Inflammation, 2013, doi:10.1155/2013/981984.
dc.description.abstractThe diagnosis of tuberculosis remains challenging in individuals with difficulty in providing good quality sputum samples such as children. Host biosignatures of inflammatory markers may be valuable in such cases, especially if they are based on more easily obtainable samples such as saliva. To explore the potential of saliva as an alternative sample in tuberculosis diagnostic/biomarker investigations, we evaluated the levels of 33 hostmarkers in saliva samples fromindividuals presenting with pulmonary tuberculosis symptoms and compared them to those obtained in serum. Of the 38 individuals included in the study, tuberculosis disease was confirmed in 11 (28.9%) by sputum culture. In both the tuberculosis cases and noncases, the levels of most markers were above the minimum detectable limit in both sample types, but there was no consistent pattern regarding the ratio ofmarkers in serum/saliva. Fractalkine, IL-17, IL-6, IL-9, MIP-1𝛽�, CRP, VEGF, and IL-5 levels in saliva and IL-6, IL-2, SAP, and SAA levels in serum were significantly higher in tuberculosis patients (𝑃� < 0.05). These preliminary data indicate that there are significant differences in the levels of host markers expressed in saliva in comparison to those expressed in serumand that inflammatory markers in both sample types are potential diagnostic candidates for tuberculosis disease.en_ZA
dc.description.sponsorshipStellenbosch Universityen_ZA
dc.description.versionPublishers' versionen_ZA
dc.format.extent10 p. : ill.
dc.identifier.citationPhalane, K. G. et al. 2013. Differential expression of host biomarkers in saliva and serum samples from individuals with suspected pulmonary tuberculosis. Mediators of Inflammation, 2013, doi:10.1155/2013/981984.en_ZA
dc.identifier.issn0962-9351 (print)
dc.identifier.issn1466-1861 (online)
dc.identifier.otherdoi:10.1155/2013/981984
dc.identifier.urihttp://hdl.handle.net/10019.1/86140
dc.language.isoen_ZAen_ZA
dc.publisherHindawi Publishing Corporationen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectTuberculosis in children -- Diagnosisen_ZA
dc.subjectSerum markersen_ZA
dc.subjectSaliva markers
dc.subjectTuberculosis in children -- Serodiagnosisen_ZA
dc.subjectSaliva samples -- Researchen_ZA
dc.subjectSerum samples -- Researchen_ZA
dc.titleDifferential expression of host biomarkers in saliva and serum samples from individuals with suspected pulmonary tuberculosisen_ZA
dc.typeArticleen_ZA
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