Modulation of inflammatory cytokines and islet morphology as therapeutic mechanisms of Basella alba in streptozotocin-induced diabetic rats
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Korean Society of Toxicology
Abstract
The mechanism of the previously reported antidiabetic effect of Basella alba is unknown. This study investigated
the role of B. alba aqueous leaf extract in the modulation of inflammatory cytokines and islet morphology in
streptozotocin-induced diabetic rats. Forty male Wistar rats, between 8 and 10 weeks old, were randomly divided
into four groups (n = 10) and administered the following treatments: Healthy control (H-c) and Diabetic control
(D-c) animals received normal saline 0.5 mL/100 g body weight daily, while Healthy Treatment (H-Ba) and Diabetic
Treatment (D-Ba) rats received the plant extract 200 mg/kg body weight daily. All treatments were administered
by oral gavage. Diabetes was induced in D-c and D-Ba rats by a single intraperitoneal injection of
streptozotocin (55 mg/kg body). The body weight and fasting blood sugar (FBS) levels were recorded every week
for 4 weeks, after which the rats were euthanized and samples collected for further analysis. After the experiment,
FBS level was significantly reduced (p < 0.0001) in rats in the D-Ba group, but increased (p < 0.001) in rats in the
D-c group. The absolute (H-c and H-Ba vs D-c, p < 0.05) and relative (D-Ba vs H-c, p < 0.05; D-Ba vs H-Ba,
p < 0.005) weights of the pancreases were significantly higher after the experiment. The rats in the D-c group had
significantly higher levels of serum interleukin-1β (p < 0.001 vs H-c; p < 0.05 vs H-Ba and D-Ba) and monocyte
chemotactic protein-1 (p < 0.0001), but lower levels of interleukin-10 (p < 0.05) in comparison with the other
groups. Histopathological examination revealed severe interstitial congestion, reduced islet area (p < 0.0001), and
increased islet cell density in the D-c group compared with those in the D-Ba group. From these findings, it was
concluded that the aqueous extract of B. alba stimulates the recovery of beta-islet morphology in streptozotocininduced
diabetic rats by modulating the peripheral production of inflammatory cytokines.
Description
CITATION: Arokoyo, D. S., et al. 2018. Modulation of inflammatory cytokines and islet morphology as therapeutic mechanisms of Basella alba in streptozotocin-induced diabetic rats. Toxicological Research, 34(4):325-332, doi:10.5487/TR.2018.34.4.325.
The original publication is available at http://www.toxicolres.org
The original publication is available at http://www.toxicolres.org
Keywords
Inflammation -- Immunological aspects, Diabetes -- Treatment, Basella alba -- Therapeutic use, Pancreas -- Cytology, Streptozotocin
Citation
Arokoyo, D. S., et al. 2018. Modulation of inflammatory cytokines and islet morphology as therapeutic mechanisms of Basella alba in streptozotocin-induced diabetic rats. Toxicological Research, 34(4):325-332, doi:10.5487/TR.2018.34.4.325