In vitro fertilization failure: Identification of gamete defects by investigation of sperm-zona pellucida binding capacity of unfertilized oocytes

Simple item page
dc.contributor.authorWindt M.-L.
dc.contributor.authorFranken D.R.
dc.contributor.authorKruger T.F.
dc.contributor.authorOehninger S.C.
dc.date.accessioned2011-05-15T16:15:27Z
dc.date.available2011-05-15T16:15:27Z
dc.date.issued1996
dc.description.abstractThe objective of the study was to determine whether fertilization failure was due to spermatozoal or oocyte factors. Twenty-five unfertilized oocytes and from 12 IVF/GIFT couples showing total or partial fertilization failure were evaluated for sperm zona binding potential under hemizona assay (HZA) conditions. Hemizonae were separately incubated with a sperm sample from the husband and that of a fertile control. Tight sperm binding to hemizonae was assessed. First, among the 12 patients, result showed a possible zona defect thought to be the cause of fertilization failure in five cases. Second, in two cases, fertilization failure was possibly caused by poor sperm binding potential of spermatozoa. Third in two cases, fertilization failure was possibly caused by an oocyte defect, and fourth, three cases showed a mixture of possible causes. The results stress the need to develop a sequential analytic programme for those couples with repeated total or partial fertilization failure.
dc.description.versionArticle
dc.identifier.citationAndrologia
dc.identifier.citation28
dc.identifier.citation4
dc.identifier.issn03034569
dc.identifier.urihttp://hdl.handle.net/10019.1/13346
dc.subjectadult
dc.subjectarticle
dc.subjectclinical article
dc.subjectclinical protocol
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectfertility
dc.subjectfertilization in vitro
dc.subjectgamete intrafallopian transfer
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectoocyte
dc.subjectpractice guideline
dc.subjectsemen analysis
dc.subjectspermatozoon abnormality
dc.subjecttreatment failure
dc.subjectzona pellucida
dc.subjectAdult
dc.subjectFemale
dc.subjectFertilization in Vitro
dc.subjectGamete Intrafallopian Transfer
dc.subjectHumans
dc.subjectInfertility
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectOocytes
dc.subjectSpermatozoa
dc.subjectZona Pellucida
dc.titleIn vitro fertilization failure: Identification of gamete defects by investigation of sperm-zona pellucida binding capacity of unfertilized oocytes
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Tygerberg Hospital Publications