Ergothioneine is a secreted antioxidant in mycobacterium smegmatis

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dc.contributor.authorEmani, Carine Saoen_ZA
dc.contributor.authorWilliams, Monique J.en_ZA
dc.contributor.authorWiid, Ian J.en_ZA
dc.contributor.authorHiten, Nicholas F.en_ZA
dc.contributor.authorViljoen, Albertus J.en_ZA
dc.contributor.authorPietersen, Ray-Dean D.en_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.contributor.authorBaker, Bienyameenen_ZA
dc.date.accessioned2013-06-13T12:18:42Z
dc.date.available2014-01-31T03:00:11Z
dc.date.issued2013-07
dc.descriptionThe original publication is available at http://aac.asm.org/en_ZA
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.
dc.description.abstractErgothioneine (ERG) and mycothiol (MSH) are two low-molecular-weight thiols synthesized by mycobacteria. The role of MSH has been extensively investigated in mycobacteria; however, little is known about the role of ERG in mycobacterial physiology. In this study, quantification of ERG at various points in the growth cycle of Mycobacterium smegmatis revealed that a significant portion of ERG is found in the culture media, suggesting that it is actively secreted. A mutant of M. smegmatis lacking egtD (MSMEG_6247) was unable to synthesize ERG, confirming its role in ERG biosynthesis. Deletion of egtD from wild-type M. smegmatis and an MSH-deficient mutant did not affect their susceptibility to antibiotics tested in this study. The ERG- and MSH-deficient double mutant was significantly more sensitive to peroxide than either of the single mutants lacking either ERG or MSH, suggesting that both thiols play a role in protecting M. smegmatis against oxidative stress and that ERG is able to partly compensate for the loss of MSH.en_ZA
dc.description.versionPublishers' versionen_ZA
dc.embargo.terms2014-01-31en_ZA
dc.format.extent12 p. : ill.
dc.identifier.citationEmani, C. S. et al. 2013. Ergothioneine is a secreted antioxidant in mycobacterium smegmatis. Antimicrob. Agents Chemother., 57(7):3202-3207, doi:10.1128/AAC.02572-12 .en_ZA
dc.identifier.issn1098-6596 (online)
dc.identifier.issn0066-4804 (print)
dc.identifier.otherdoi:10.1128/AAC.02572-12
dc.identifier.urihttp://hdl.handle.net/10019.1/80919
dc.language.isoen_ZAen_ZA
dc.publisherAmerican Society for Microbiologyen_ZA
dc.rights.holderAmerican Society for Microbiologyen_ZA
dc.subjectErgothioneineen_ZA
dc.subjectMycobacterium smegmatis -- Effect of oxidative stress onen_ZA
dc.subjectBiosynthesisen_ZA
dc.subjectOxidative stressen_ZA
dc.subjectAntioxidant status of cellsen_ZA
dc.titleErgothioneine is a secreted antioxidant in mycobacterium smegmatisen_ZA
dc.typeArticleen_ZA
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