Microsatellite-based DNA fingerprinting of selected grapevine cultivars
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
South African Society for Enology and Viticulture
Abstract
Cultivar identification by ampelography is often difficult and is sensitive to environmental conditions, thus it can be problematic to distinguish between closely related cultivars. DNA fingerprinting offers an alternative method that is not influenced by the environment and is relatively easy to perform. However, discriminating between closely related individuals can be problematic if inadequate or insufficient markers are used. Following the estimation of null allele frequencies, an initial set of 35 microsatellite markers was reduced to 20 to generate unique DNA fingerprints for the majority of 111 different grape cultivars and breeding lines. Molecular evidence was utilised to evaluate the accuracy of the reported pedigrees for several cultivars bred in South Africa (SA). The use of markers linked to known downy mildew and powdery mildew resistance loci (Rpv3 and Ren3) provided information regarding the frequency of these resistance loci in the breeding material analysed.
Description
CITATION: Van Heerden, C. J., Burger, P. & Prins, R. 2018. Microsatellite-based DNA fingerprinting of selected grapevine cultivars. South African Journal of Enology & Viticulture, 39(1):58-66, doi:10.21548/39-1-2053.
The original publication is available at http://www.journals.ac.za/index.php/sajev
The original publication is available at http://www.journals.ac.za/index.php/sajev
Keywords
Cultivar identification, Vitis vinifera, Grapevine cultivars, DNA fingerprinting
Citation
Van Heerden, C. J., Burger, P. & Prins, R. 2018. Microsatellite-based DNA fingerprinting of selected grapevine cultivars. South African Journal of Enology & Viticulture, 39(1):58-66, doi:10.21548/39-1-2053