Use of a zona laser treatment system in assisted hatching: Optimal laser utilization parameters

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dc.contributor.authorTinney G.M.
dc.contributor.authorWindt M.-L.
dc.contributor.authorKruger T.F.
dc.contributor.authorLombard C.J.
dc.date.accessioned2011-05-15T16:16:25Z
dc.date.available2011-05-15T16:16:25Z
dc.date.issued2005
dc.description.abstractObjective: This study aimed to establish the benefit of laser micromanipulation of the zona pellucida as a method of assisted hatching and to determine the optimal technical settings. Design: Prospective pilot study. Setting: University teaching hospital. Animal(s): Six- to 8-week-old F1 female mice. Intervention(s): Mouse embryos were treated with a laser (Hamilton Thorne Research) using both research and preset clinical settings. The research option allows the user to alter pulse duration, number of pulses, and beam intensity. In this study, laser intensity and duration, as well as the depth and number of disruptions, were altered. Main Outcome Measure(s): Embryos were scored after treatment as either arrested blastocysts, arrested during hatching, hatched, or degenerate. Result(s): The treatment where the laser intensity was halved but the number of laser pulses used was doubled was the most beneficial treatment. Zona thinning was not effective. Conclusion(s): The study showed that laser-assisted hatching can safely and significantly increase the percentage of embryos reaching the hatched blastocyst stage. A proportionately sized hole in a human embryo may have the same beneficial effect as has been shown in this study. ©2005 by American Society for Reproductive Medicine.
dc.description.versionArticle
dc.identifier.citationFertility and Sterility
dc.identifier.citation84
dc.identifier.citation6
dc.identifier.issn00150282
dc.identifier.other10.1016/j.fertnstert.2005.05.048
dc.identifier.urihttp://hdl.handle.net/10019.1/13775
dc.subjectanimal experiment
dc.subjectarticle
dc.subjectblastocyst
dc.subjectcontrolled study
dc.subjectembryo
dc.subjectfemale
dc.subjecthatching
dc.subjectinfertility therapy
dc.subjectlaser
dc.subjectmedical research
dc.subjectmicromanipulation
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectscoring system
dc.subjectstatistical significance
dc.subjectzona pellucida
dc.subjectAnimals
dc.subjectBlastocyst
dc.subjectFemale
dc.subjectLasers
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMice, Inbred C57BL
dc.subjectMicromanipulation
dc.subjectModels, Animal
dc.subjectPilot Projects
dc.subjectPregnancy
dc.subjectReproductive Techniques, Assisted
dc.subjectZona Pellucida
dc.titleUse of a zona laser treatment system in assisted hatching: Optimal laser utilization parameters
dc.typeArticle
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