Mycobacterium tuberculosis Beijing genotype is associated with HIV infection in Mozambique

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dc.contributor.authorViegas, Sofia O.en_ZA
dc.contributor.authorMachado, Adelinaen_ZA
dc.contributor.authorGroenheit, Ramonaen_ZA
dc.contributor.authorGhebremichel, Solomonen_ZA
dc.contributor.authorPennhag, Alexandraen_ZA
dc.contributor.authorGudo, Paula S.en_ZA
dc.contributor.authorCuna, Zainaen_ZA
dc.contributor.authorLanga, Egídioen_ZA
dc.contributor.authorMiotto, Pauloen_ZA
dc.contributor.authorCirillo, Daniela M.en_ZA
dc.contributor.authorRastogi, Nalinen_ZA
dc.contributor.authorWarren, Roben_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.contributor.authorKoivula, Tuijaen_ZA
dc.contributor.authorKullenius, Gunillaen_ZA
dc.date.accessioned2014-07-07T12:18:21Z
dc.date.available2014-07-07T12:18:21Z
dc.date.issued2013-08-07
dc.descriptionCITATION: Viegas, S. O. et al. 2013. Mycobacterium tuberculosis Beijing genotype is associated with HIV infection in Mozambique. PLoS ONE, 8(8): e71999, doi:10.1371/journal.pone.0071999.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractThe Beijing genotype is a lineage of Mycobacterium tuberculosis that is distributed worldwide and responsible for large epidemics, associated with multidrug-resistance. However, its distribution in Africa is less understood due to the lack of data. Our aim was to investigate the prevalence and possible transmission of Beijing strains in Mozambique by a multivariate analysis of genotypic, geographic and demographic data. A total of 543 M. tuberculosis isolates from Mozambique were spoligotyped. Of these, 33 were of the Beijing lineage. The genetic relationship between the Beijing isolates were studied by identification of genomic deletions within some Regions of Difference (RD), Restriction Fragment Length Polymorphism (RFLP) and Mycobacterial Interspersed Repetivie Unit – variable number tandem repeat (MIRU-VNTR). Beijing strains from South Africa, representing different sublineages were included as reference strains. The association between Beijing genotype, Human Immunodeficiency Virus (HIV) serology and baseline demographic data was investigated. HIV positive serostatus was significantly (p=0.023) more common in patients with Beijing strains than in patients with non-Beijing strains in a multivariable analysis adjusted for age, sex and province (14 (10.9%) of the 129 HIV positive patients had Beijing strains while 6/141 (4.3%) of HIV negative patients had Beijing strains). The majority of Beijing strains were found in the Southern region of Mozambique, particularly in Maputo City (17%). Only one Beijing strain was drug resistant (multi-drug resistant). By combined use of RD and spoligotyping, three genetic sublineages could be tentatively identified where a distinct group of four isolates had deletion of RD150, a signature of the “sublineage 7” recently emerging in South Africa. The same group was very similar to South African “sublineage 7” by RFLP and MIRU-VNTR, suggesting that this sublineage could have been recently introduced in Mozambique from South Africa, in association with HIV infection.
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0071999
dc.description.versionPublisher's version
dc.format.extent9 pages
dc.identifier.citationViegas, S. O. et al. 2013. Mycobacterium tuberculosis Beijing genotype is associated with HIV infection in Mozambique. PLoS ONE, 8(8): e71999, doi:10.1371/journal.pone.0071999.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0071999
dc.identifier.urihttp://hdl.handle.net/10019.1/93335
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectMycobacterium tuberculosis -- Mozambiqueen_ZA
dc.subjectHIV infections -- Mozambiqueen_ZA
dc.titleMycobacterium tuberculosis Beijing genotype is associated with HIV infection in Mozambiqueen_ZA
dc.typeArticle
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