Directly interrogating single quantum dot labelled UvrA2 molecules on DNA tightropes using an optically trapped nanoprobe

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dc.contributor.authorSimons, Michelleen_ZA
dc.contributor.authorPollard, Mark R.en_ZA
dc.contributor.authorHughes, Craig D.en_ZA
dc.contributor.authorWard, Andrew D.en_ZA
dc.contributor.authorVan Houten, Bennetten_ZA
dc.contributor.authorTowrie, Mikeen_ZA
dc.contributor.authorBotchway, Stan W.en_ZA
dc.contributor.authorParker, Anthony W.en_ZA
dc.contributor.authorKad, Neil M.en_ZA
dc.date.accessioned2017-10-20T13:07:32Z
dc.date.available2017-10-20T13:07:32Z
dc.date.issued2016
dc.descriptionCITATION: Simons, M., et al. 2016. Directly interrogating single quantum dot labelled UvrA2 molecules on DNA tightropes using an optically trapped nanoprobe. Scientific Reports, 5:18486, doi:10.1038/srep18486.en_ZA
dc.descriptionThe original publication is available at http://www.nature.com/srepen_ZA
dc.description.abstractIn this study we describe a new methodology to physically probe individual complexes formed between proteins and DNA. By combining nanoscale, high speed physical force measurement with sensitive fluorescence imaging we investigate the complex formed between the prokaryotic DNA repair protein UvrA2 and DNA. This approach uses a triangular, optically-trapped “nanoprobe” with a nanometer scale tip protruding from one vertex. By scanning this tip along a single DNA strand suspended between surface-bound micron-scale beads, quantum-dot tagged UvrA2 molecules bound to these ‘”DNA tightropes” can be mechanically interrogated. Encounters with UvrA2 led to deflections of the whole nanoprobe structure, which were converted to resistive force. A force histogram from all 144 detected interactions generated a bimodal distribution centered on 2.6 and 8.1 pN, possibly reflecting the asymmetry of UvrA2’s binding to DNA. These observations successfully demonstrate the use of a highly controllable purpose-designed and built synthetic nanoprobe combined with fluorescence imaging to study protein-DNA interactions at the single molecule level.en_ZA
dc.description.urihttps://www.nature.com/articles/srep18486
dc.description.versionPublisher's versionen_ZA
dc.format.extent9 pages : illustrations (some colour)en_ZA
dc.identifier.citationSimons, M., et al. 2016. Directly interrogating single quantum dot labelled UvrA2 molecules on DNA tightropes using an optically trapped nanoprobe. Scientific Reports, 5:18486, doi:10.1038/srep18486en_ZA
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/srep18486
dc.identifier.urihttp://hdl.handle.net/10019.1/102365
dc.language.isoen_ZAen_ZA
dc.publisherSpringer Natureen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectDNA-protein interactionsen_ZA
dc.subjectMolecular biology -- Researchen_ZA
dc.subjectDNA -- Researchen_ZA
dc.titleDirectly interrogating single quantum dot labelled UvrA2 molecules on DNA tightropes using an optically trapped nanoprobeen_ZA
dc.typeArticleen_ZA
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