Involvement of Fenton chemistry in rice straw degradation by the lignocellulolytic bacterium Pantoea ananatis Sd-1

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dc.contributor.authorMa, Jiangshanen_ZA
dc.contributor.authorZhang, Kekeen_ZA
dc.contributor.authorHuang, Meien_ZA
dc.contributor.authorHector, Stanton B.en_ZA
dc.contributor.authorLiu, Binen_ZA
dc.contributor.authorTong, Chunyien_ZA
dc.contributor.authorLiu, Qianen_ZA
dc.contributor.authorZeng, Jiaruien_ZA
dc.contributor.authorGao, Yanen_ZA
dc.contributor.authorXu, Tingen_ZA
dc.contributor.authorLiu, Yingen_ZA
dc.contributor.authorLiu, Xuanmingen_ZA
dc.contributor.authorZhu, Yonghuaen_ZA
dc.date.accessioned2017-01-24T10:58:30Z
dc.date.available2017-01-24T10:58:30Z
dc.date.issued2016
dc.date.updated2016-12-09T12:08:41Z
dc.descriptionCITATION: Ma, J., et al. 2016. Involvement of Fenton chemistry in rice straw degradation by the lignocellulolytic bacterium Pantoea ananatis Sd-1. Biotechnol Biofuels, 9:211, doi: 10.1186/s13068-016-0623-x.en_ZA
dc.descriptionThe original publication is available at https://biotechnologyforbiofuels.biomedcentral.comen_ZA
dc.description.abstractBackground: Lignocellulolytic bacteria have revealed to be a promising source for biofuel production, yet the underlying mechanisms are still worth exploring. Our previous study inferred that the highly efficient lignocellulose degradation by bacterium Pantoea ananatis Sd-1 might involve Fenton chemistry (Fe2+ + H2O2 + H+ → Fe3+ + OH · + H2O), similar to that of white-rot and brown-rot fungi. The aim of this work is to investigate the existence of this Fenton-based oxidation mechanism in the rice straw degradation process of P. ananatis Sd-1. Results: After 3 days incubation of unpretreated rice straw with P. ananatis Sd-1, the percentage in weight reduction of rice straw as well as its cellulose, hemicellulose, and lignin components reached 46.7, 43.1, 42.9, and 37.9 %, respectively. The addition of different hydroxyl radical scavengers resulted in a significant decline (P < 0.001) in rice straw degradation. Pyrolysis gas chromatography–mass spectrometry and Fourier transform infrared spectroscopy analysis revealed the consistency of chemical changes of rice straw components that exists between P. ananatis Sd-1 and Fenton reagent treatment. In addition to the increased total iron ion concentration throughout the rice straw decomposition process, the Fe3+-reducing capacity of P. ananatis Sd-1 was induced by rice straw and predominantly contributed by aromatic compounds metabolites. The transcript levels of the glucose-methanol-choline oxidoreductase gene related to hydrogen peroxide production were significantly up-regulated (at least P < 0.01) in rice straw cultures. Higher activities of GMC oxidoreductase and less hydrogen peroxide concentration in rice straw cultures relative to glucose cultures may be responsible for increasing rice straw degradation, which includes Fenton-like reactions. Conclusions: Our results confirmed the Fenton chemistry-assisted degradation model in P. ananatis Sd-1. We are among the first to show that a Fenton-based oxidation mechanism exists in a bacteria degradation system, which provides a new perspective for how natural plant biomass is decomposed by bacteria. This degradative system may offer an alternative approach to the fungi system for lignocellulosic biofuels production.en_ZA
dc.description.sponsorshipNational Natural Science Foundation of China (51378191).en_ZA
dc.description.urihttps://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0623-xen_ZA
dc.description.versionPublisher's versionen_ZA
dc.format.extent13 pages : illustrationsen_ZA
dc.identifier.citationMa, J., et al. 2016. Involvement of Fenton chemistry in rice straw degradation by the lignocellulolytic bacterium Pantoea ananatis Sd-1. Biotechnol Biofuels, 9:211, doi:10.1186/s13068-016-0623-xen_ZA
dc.identifier.issn1754-6834(Online)
dc.identifier.otherdoi:10.1186/s13068-016-0623-x
dc.identifier.urihttp://hdl.handle.net/10019.1/100511
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Centralen_ZA
dc.rights.holderAuthor retains copyrighten_ZA
dc.subjectLignocellulose degradationen_ZA
dc.subjectPantoea ananatisen_ZA
dc.subjectFenton-based oxidation mechanismen_ZA
dc.subjectRice straw degradation processen_ZA
dc.titleInvolvement of Fenton chemistry in rice straw degradation by the lignocellulolytic bacterium Pantoea ananatis Sd-1en_ZA
dc.typeArticleen_ZA
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