A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1)

Simple item page
dc.contributor.advisorSwart, Amanda C.en_ZA
dc.contributor.advisorSwart, P.en_ZA
dc.contributor.authorStorbeck, Karl-Heinzen_ZA
dc.contributor.otherUniversity of Stellenbosch. Faculty of Science. Dept. of Biochemistry.en_ZA
dc.date.accessioned2006-11-15T09:02:30Zen_ZA
dc.date.accessioned2010-06-01T09:06:37Z
dc.date.available2006-11-15T09:02:30Zen_ZA
dc.date.available2010-06-01T09:06:37Z
dc.date.issued2005-12
dc.descriptionThesis (MSc (Biochemistry))--University of Stellenbosch, 2005.en_ZA
dc.description.abstractThis study describes: 1. The cloning of baboon cytochrome P450 side-chain cleavage (CYP11A1) cDNA by in vitro site-directed mutagenesis. 2. The identification and sequencing of three baboon CYP11A1 mutants: CYP11A1a, CYP11A1b and CYP11A1c. 3. The expression and characterisation of baboon and human CYP11A1 cDNA, CYP11A1a, CYP11A1b and CYP11A1c in nonsteroidogenic COS-1 cells. The Km and V-values for the metabolism of 25-hydroxycholesterol were determined. 4. The construction of the first homology model of CYP11A1, using both mammalian (CYP2C5) and bacterial (CYP102) cytochromes P450 crystal structures as templates. 5. A structure/function study into the role of the amino acid residues Ile98, Lys103 and Thr291 in substrate binding and enzymatic activity. 6. The proposal of a topological model of the CYP11A1 active pocket as determined by substrate docking studies.en_ZA
dc.format.extent7447068 bytesen_ZA
dc.format.mimetypeapplication/pdfen_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/3113
dc.language.isoen
dc.publisherStellenbosch : University of Stellenboschen_ZA
dc.rights.holderUniversity of Stellenboschen_ZA
dc.subjectDissertations -- Biochemistryen_ZA
dc.subjectTheses -- Biochemistryen_ZA
dc.subjectCytochrome P-450en_ZA
dc.subjectMutagenesisen_ZA
dc.subjectAdrenocortical hormoneen_ZA
dc.subjectHomology (Biology)en_ZA
dc.subjectAmino acidsen_ZA
dc.subjectBaboons -- Cytogeneticsen_ZA
dc.subjectMolecular cloningen_ZA
dc.titleA structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1)en_ZA
dc.typeThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
storbeck_structure_2005.pdf
Size:
7.1 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.72 KB
Format:
Plain Text
Description:
Download
Collections
Masters Degrees (Biochemistry)