Molecular characterisation of Mycobacterium bovis isolated from African buffaloes (Syncerus caffer) in Hluhluwe-iMfolozi Park in KwaZulu-Natal, South Africa

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dc.contributor.authorHlokwe, Tiny M.en_ZA
dc.contributor.authorJenkins, Akinbowale O.en_ZA
dc.contributor.authorStreicher, Elizabeth M.en_ZA
dc.contributor.authorVenter, Estelle H.en_ZA
dc.contributor.authorCooper, Daveen_ZA
dc.contributor.authorGodfroid, Jacquesen_ZA
dc.contributor.authorMichel, Anita L.en_ZA
dc.date.accessioned2012-01-31T15:05:55Z
dc.date.available2012-01-31T15:05:55Z
dc.date.issued2011
dc.descriptionCITATION: Hlokwe, T. M., et al. 2011. Molecular characterisation of Mycobacterium bovis isolated from African buffaloes (Syncerus caffer) in Hluhluwe-iMfolozi Park in KwaZulu-Natal, South Africa. Onderstepoort Journal of Veterinary Research, 78(1), Art. #232, doi:10.4102/ojvr.v78i1.232.en_ZA
dc.descriptionThe original publication is available at http://www.ojvr.orgen_ZA
dc.description.abstractBovine tuberculosis (BTB), a chronic disease of mammals caused by Mycobacterium bovis, is a threat to South African wildlife. It has been reported that African buffaloes (Syncerus caffer) are reservoir hosts of BTB in South African wildlife populations. This study reports on the molecular identification and typing of 31 M. bovis isolates collected between 1993 and 2008, mainly from buffaloes but also from two lions and a bush pig, in the Hluhluwe-iMfolozi Park (HiP) in KwaZulu-Natal. To study the dynamics of BTB in the buffalo populations, 28 M. bovis isolates from the HiP and epidemiologically related parks were characterised using regions of difference deletion analysis for species identification and spoligotyping, variable number of tandem repeats (VNTR), polymorphic G–C-rich sequences and IS6110 restriction fragment length polymorphism (RFLP) genotyping methods. At least three distinct M. bovis genotypes were found amongst HiP samples. The combination of VNTR typing (using a 16-loci panel) and IS6110 RFLP revealed the presence of three additional genetic profiles in individual buffaloes, demonstrating that the highest level of discrimination was achieved by these typing methods. One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates from buffaloes. A novel M. bovis spoligotype (SB1474), which is reported for the first time in this study, was observed in 14.3% of isolates from buffaloes. Based on the observed genetic relationships, the findings suggest independent introductions from at least three unrelated sources. These findings improve the knowledge regarding the diversity of circulating M. bovis strains in the HiP.en_ZA
dc.description.urihttp://www.ojvr.org/index.php/ojvr/article/view/232
dc.description.versionPublisher's versionen_ZA
dc.format.extent6 pages : illustrations (some colour), mapsen_ZA
dc.identifier.citationHlokwe, T. M., et al. 2011. Molecular characterisation of Mycobacterium bovis isolated from African buffaloes (Syncerus caffer) in Hluhluwe-iMfolozi Park in KwaZulu-Natal, South Africa. Onderstepoort Journal of Veterinary Research, 78(1), Art. #232, doi:10.4102/ojvr.v78i1.232en_ZA
dc.identifier.issn2219-0635 (online)
dc.identifier.issn0030-2465 (print)
dc.identifier.other10.4102/ojvr.v78i1.232
dc.identifier.otherdoi:10.4102/ojvr.v78i1.232
dc.identifier.urihttp://hdl.handle.net/10019.1/19430
dc.language.isoen_ZAen_ZA
dc.publisherAOSIS Publishingen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectTuberculosis in buffaloes -- Kwa-Zulu-Natal (South Africa)en_ZA
dc.subjectWildlife diseases -- Molecular aspectsen_ZA
dc.subjectMycobacterium bovisen_ZA
dc.titleMolecular characterisation of Mycobacterium bovis isolated from African buffaloes (Syncerus caffer) in Hluhluwe-iMfolozi Park in KwaZulu-Natal, South Africaen_ZA
dc.typeArticleen_ZA
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