Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes

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dc.contributor.authorDuong, Yen T.en_ZA
dc.contributor.authorKassanjee, Reshmaen_ZA
dc.contributor.authorWelte, Alexen_ZA
dc.contributor.authorMorgan, Meadeen_ZA
dc.contributor.authorDe, Anindyaen_ZA
dc.contributor.authorDobbs, Trudyen_ZA
dc.contributor.authorRottinghaus, Erinen_ZA
dc.contributor.authorNkengasong, Johnen_ZA
dc.contributor.authorKittinunvorakoon, Chontichaen_ZA
dc.contributor.authorRaengsakulrach, Boonyosen_ZA
dc.contributor.authorMartin, Michaelen_ZA
dc.contributor.authorChoopanya, Kachiten_ZA
dc.contributor.authorVanichseni, Suphaken_ZA
dc.contributor.authorJiang, Yanen_ZA
dc.contributor.authorQiu, Maofengen_ZA
dc.contributor.authorYu, Haiyingen_ZA
dc.contributor.authorHao, Yanen_ZA
dc.contributor.authorShah, Nehaen_ZA
dc.contributor.authorLe, Linh-Vien_ZA
dc.contributor.authorKim, Andrea A.en_ZA
dc.contributor.authorNguyen, Tuan Anhen_ZA
dc.contributor.authorAmpofo, Williamen_ZA
dc.contributor.authorParekh, Bharat S.en_ZA
dc.contributor.authorCurlin, Marcel E.en_ZA
dc.date.accessioned2016-08-30T10:34:49Z
dc.date.available2016-08-30T10:34:49Z
dc.date.issued2015
dc.descriptionCITATION: Duong, Y. T., et al. 2015. Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes. PLoS ONE, 10(2):1-15, doi:10.1371/journal.pone.0114947.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractBackground: Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that MDRI of LAg-Avidity EIA estimated previously required recalibration. We present here results of recalibration efforts using >250 seroconversion panels and multiple statistical methods to ensure accuracy and consensus. Methods: A total of 2737 longitudinal specimens collected from 259 seroconverting individuals infected with diverse HIV-1 subtypes were tested with the LAg-Avidity EIA as previously described. Data were analyzed for determination of MDRI at ODn cutoffs of 1.0 to 2.0 using 7 statistical approaches and sub-analyzed by HIV-1 subtypes. In addition, 3740 specimens from individuals with infection >1 year, including 488 from patients with AIDS, were tested for PFR at varying cutoffs. Results: Using different statistical methods, MDRI values ranged from 88–94 days at cutoff ODn = 1.0 to 177–183 days at ODn = 2.0. The MDRI values were similar by different methods suggesting coherence of different approaches. Testing for misclassification among long-term infections indicated that overall PFRs were 0.6% to 2.5% at increasing cutoffs of 1.0 to 2.0, respectively. Balancing the need for a longer MDRI and smaller PFR (<2.0%) suggests that a cutoff ODn = 1.5, corresponding to an MDRI of 130 days should be used for cross-sectional application. The MDRI varied among subtypes from 109 days (subtype A&D) to 152 days (subtype C). Conclusions: Based on the new data and revised analysis, we recommend an ODn cutoff = 1.5 to classify recent and long-term infections, corresponding to an MDRI of 130 days (118–142). Determination of revised parameters for estimation of HIV-1 incidence should facilitate application of the LAg-Avidity EIA for worldwide useen_ZA
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114947
dc.description.versionPublisher's versionen_ZA
dc.format.extent15 pagesen_ZA
dc.identifier.citationDuong, Y. T., et al. 2015. Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes. PLoS ONE, 10(2):1-15, doi:10.1371/journal.pone.0114947
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0114947
dc.identifier.urihttp://hdl.handle.net/10019.1/99509
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectMean duration of recent infectionen_ZA
dc.subjectHIV infections -- Diagnosisen_ZA
dc.titleRecalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypesen_ZA
dc.typeArticleen_ZA
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