Detection of human herpes virus 8 DNA and sequence polymorphism in classical, epidemic, and iatrogenic Kaposi's sarcoma in South Africa

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dc.contributor.authorEngelbrecht S.
dc.contributor.authorTreurnicht F.K.
dc.contributor.authorSchneider J.W.
dc.contributor.authorJordaan H.F.
dc.contributor.authorSteytler J.G.
dc.contributor.authorWranz P.A.B.
dc.contributor.authorVan Rensburg E.J.
dc.date.accessioned2011-05-15T16:17:03Z
dc.date.available2011-05-15T16:17:03Z
dc.date.issued1997
dc.description.abstractThe aetiology and detection of human herpes virus type 8 (HHV-8) DNA sequences in Kaposi's sarcoma (KS) is a matter of intense investigation. We report on the detection of HHV-8 DNA and sequence polymorphism in different clinicopathological subtypes of cutaneous KS samples from South Africa. The diagnosis was confirmed by histological examination in all cases. Six patients had classic KS (CKS), 3 epidemic KS (EKS), and 3 iatrogenic KS (IKS). A nested polymerase chain reaction (PCR) assay was used to detect HHV-8 DNA in cell lysates, prepared from formalin fixed, paraffin embedded sections. We investigated polymorphism in the HHV-8 DNA using single-stranded conformational polymorphism (SSCP) analysis on the PCR products, followed by direct sequencing. HHV-8 DNA was detected in all the patients with KS, irrespective of the clinicopathological subtype. Direct sequencing was performed on 5 selected cases and showed single base pair substitutions in all. The spectrum of mutations was similar to those described previously. No correlation was found between the different types of KS and sequence variation. The results support the hypothesis that HHV-8 is strongly associated with different clinicopathological subtypes of KS and confirm the occurrence of HHV-8 in patients with CKS, EKS, and IKS in South Africa.
dc.description.versionArticle
dc.identifier.citationJournal of Medical Virology
dc.identifier.citation52
dc.identifier.citation2
dc.identifier.issn01466615
dc.identifier.other10.1002/(SICI)1096-9071(199706)52:2<168::AID-JMV9>3.0.CO;2-1
dc.identifier.urihttp://hdl.handle.net/10019.1/14052
dc.subjectarticle
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectgenetic polymorphism
dc.subjecthuman
dc.subjecthuman herpesvirus 8
dc.subjecthuman tissue
dc.subjectiatrogenic disease
dc.subjectkaposi sarcoma
dc.subjectpolymerase chain reaction
dc.subjectsouth africa
dc.subjectvirus detection
dc.subjectDisease Outbreaks
dc.subjectDNA, Viral
dc.subjectHerpesvirus 8, Human
dc.subjectHumans
dc.subjectIatrogenic Disease
dc.subjectPolymerase Chain Reaction
dc.subjectPolymorphism, Genetic
dc.subjectPolymorphism, Single-Stranded Conformational
dc.subjectSarcoma, Kaposi
dc.subjectSequence Analysis, DNA
dc.subjectSouth Africa
dc.subjectHerpes
dc.subjectherpes virus 8
dc.subjectherpes virus type 8
dc.subjectHerpesviridae
dc.subjectherpesvirus 8
dc.subjecthuman herpes virus
dc.subjecthuman herpes virus 8
dc.subjectHuman herpesvirus 8
dc.titleDetection of human herpes virus 8 DNA and sequence polymorphism in classical, epidemic, and iatrogenic Kaposi's sarcoma in South Africa
dc.typeArticle
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