Comparison of commercial assays and two-step approach to detect Clostridioides difficile in South Africa

Simple item page
dc.contributor.authorSingh, Sarishnaen_ZA
dc.contributor.authorNewton-Foot, Maeen_ZA
dc.contributor.authorNel, Pieteren_ZA
dc.contributor.authorPienaar, Coletteen_ZA
dc.date.accessioned2022-10-14T13:38:10Zen_ZA
dc.date.available2022-10-14T13:38:10Zen_ZA
dc.date.issued2022-09en_ZA
dc.descriptionCITATION: Sarishna Singh, Mae Newton-Foot, Pieter Nel, Colette Pienaar. 2022. Comparison of commercial assays and two-step approach to detect Clostridioides difficile in South Africa. African Journal of Laboratory Medicine, (11):1, a1809, doi:10.4102/ajlm.v11i1.1809.en_ZA
dc.descriptionThe original publication is available at https://ajlmonline.orgen_ZA
dc.description.abstractBackground: Clostridioides difficile is the number one cause of hospital-acquired diarrhoea. Accurate diagnosis of C. difficile is of utmost importance as it guides patient management and infection control practices. Studies evaluating the performance of commercially available nucleic acid amplification tests (NAATs) versus algorithms are lacking in resource-limited settings. Objective: This study assessed the performance of three commercially available tests and a two-step approach for the diagnosis of C. difficile infection using toxigenic culture (TC) as the gold standard. Methods: Two hundred and twenty-three non-duplicate loose stool samples were submitted to the National Health Laboratory Service Microbiology Laboratory at Tygerberg Hospital, Cape Town, South Africa, from October 2017 to October 2018. The samples were tested in parallel using the C. DIFF QUIK CHEK COMPLETE enzyme immunoassay (EIA) and two NAATs (Xpert C. difficile and BD MAX Cdiff), and the results were compared to TC. The performance of a two-step approach consisting of the C. DIFF QUIK CHEK COMPLETE followed by the Xpert C. difficile was also determined. Results: Of 223 faecal specimens tested, 37 (16.6%) were TC-positive. The sensitivity and specificity of the C. DIFF QUIK CHEK COMPLETE were 54.1% and 98.9%; Xpert C. difficile, 86.4% and 96.8%; BD MAX Cdiff, 89.2% and 96.8%; and two-step approach, 89.2% and 96.2%. Conclusion: The C. DIFF QUIK CHEK COMPLETE, in a two-step approach with the Xpert C. difficile, performed similarly to the NAATs on their own and offer advantages in terms of cost and workflow in low-resource settings.en_ZA
dc.description.urihttps://ajlmonline.org/index.php/ajlm/article/view/1809en_ZA
dc.description.versionPublisher's versionen_ZA
dc.format.extent6 pagesen_ZA
dc.identifier.citationSarishna Singh, Mae Newton-Foot, Pieter Nel, Colette Pienaar. 2022. Comparison of commercial assays and two-step approach to detect Clostridioides difficile in South Africa. African Journal of Laboratory Medicine, (11):1, a1809, doi:10.4102/ajlm.v11i1.1809.en_ZA
dc.identifier.issn2225-2010 (online)en_ZA
dc.identifier.issn2225-2002 (print)en_ZA
dc.identifier.otherDOI no:10.4102/ajlm.v11i1.1809en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/125771en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherAOSISen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectClostridium difficile -- South Africaen_ZA
dc.subjectNosocomial infections -- South Africaen_ZA
dc.subjectToxigenic cultureen_ZA
dc.subjectInfection controlen_ZA
dc.titleComparison of commercial assays and two-step approach to detect Clostridioides difficile in South Africaen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
singh_comparison_2022.pdf
Size:
653.48 KB
Format:
Adobe Portable Document Format
Description:
Download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Medical Microbiology)