Efficiency of co-expression of transcription factors Pdx1, Ngn3, NeuroD and Pax6 with insulin : a statistical approach
Date
2015-05
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Aim: The objective of this study was to investigate the time related profile and efficiency
of co-expression of the homeodomain proteins Pdx1, NeuroD, Ngn3, Pax6 and caspase3 with insulin,
and to establish the time periods post PDL optimum for islets transplantation.
Study design/methods: In this experimental study, immunofluorescent staining procedure was performed
on deparaffinized pancreatic duct ligated (PDL) tissues of 78 Sprague–Dawley rats. Quantification
of protein coexpression was made using a computerized morphometry. The efficiency of
co-expression was arbitrary defined by the value of mean ratio (score without unit) of insulin
expression divided by each expression index of the other proteins, occurring within the time interval
of 12–24 h post PDL. Statistical tool was used to analyze the efficiency of co-expression of proteins;
analysis of variances (one way ANOVA) was used to compare the means of co-expression indexes
across the time periods pre- and post PDL. P-values less than 0.05 were considered statistically significant;
no post hoc test was done.
Description
CITATION: Du Toit, DF, Longo-Mbenza, B, Page, BJ, & Tchokonte-Nana, V. 2015. Efficiency of co-expression of transcription factors Pdx1, Ngn3, NeuroD and Pax6 with insulin: A statistical approach, 3(1):57-64. https://doi.org/10.1016/j.ijdm.2011.01.007.
The original publication is available https://www.sciencedirect.com/journal/international-journal-of-diabetes-mellitus
The original publication is available https://www.sciencedirect.com/journal/international-journal-of-diabetes-mellitus
Keywords
Insulin -- Effectiveness, Islands of Langerhans -- Transplantation -- Effectiveness
Citation
Du Toit, DF, Longo-Mbenza, B, Page, BJ, & Tchokonte-Nana, V. 2015. Efficiency of co-expression of transcription factors Pdx1, Ngn3, NeuroD and Pax6 with insulin: A statistical approach. International journal of diabetes mellitus, 3(1):57-64. https://doi.org/10.1016/j.ijdm.2011.01.007.