Phylogenetic analysis of leuconostoc and lactobacillus species isolated from sugarcane processing streams

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dc.contributor.authorNel, Saneten_ZA
dc.contributor.authorDavis, Stephen B.en_ZA
dc.contributor.authorEndo, Akihitoen_ZA
dc.contributor.authorDicks, Leon Milner Theodore, 1961-en_ZA
dc.date.accessioned2020-07-28T07:07:53Z
dc.date.available2020-07-28T07:07:53Z
dc.date.issued2020
dc.descriptionCITATION: Nel, S., et al. 2020. Phylogenetic analysis of leuconostoc and lactobacillus species isolated from sugarcane processing streams. MicrobiologyOpen, 9(8):e1065, doi:10.1002/mbo3.1065.
dc.descriptionThe original publication is available at https://onlinelibrary.wiley.com
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund
dc.description.abstractHigh levels of gums such as dextran, produced by Leuconostoc and Lactobacillus spp., have a severe impact on factory throughput and sugar quality. This study aimed to determine the phylogenetic relationships between gum‐producing Leuconostoc and Lactobacillus bacteria which were isolated from various locations in a sugarcane processing factory at times when low‐ and high‐dextran raw sugar, respectively, were produced. Phylogenetic analysis of 16S rRNA gene sequences grouped 81 isolates with the type strains of Leuconostoc mesenteroides (subspp. mesenteroides , dextranicum, and cremoris ), Leuconostoc pseudomesenteroides, Leuconostoc lactis, and Leuconostoc citreum , respectively. Forty‐three isolates clustered with the type strain of Lactobacillus fermentum . The phylogenetic relatedness of the isolates was determined by sequencing and analysis of the housekeeping genes rpoA and dnaA for Leuconostoc spp. and the pheS and tuf genes for the Lactobacillus spp. The rpoA gene proved discriminatory for the phylogenetic resolution of all of the isolated Leuconostoc spp. and the dnaA housekeeping gene was shown to be effective for isolates clustering with the type strains of Leuc. mesenteroides and Leuc. citreum . None of the loci examined permitted differentiation at the subspecies level of Leuc. mesenteroides . Single‐locus analysis, as well as the concatenation of the pheS and tuf housekeeping gene sequences, yielded identical phylogenies for the Lactobacillus isolates corresponding to L. fermentum.en_ZA
dc.description.urihttps://onlinelibrary.wiley.com/doi/abs/10.1002/mbo3.1065
dc.description.versionPublisher's version
dc.format.extent10 pages
dc.identifier.citationNel, S., et al. 2020. Phylogenetic analysis of leuconostoc and lactobacillus species isolated from sugarcane processing streams. MicrobiologyOpen, 9(8):e1065, doi:10.1002/mbo3.1065
dc.identifier.issn2045-8827 (online)
dc.identifier.otherdoi:10.1002/mbo3.1065
dc.identifier.urihttp://hdl.handle.net/10019.1/108738
dc.language.isoen_ZAen_ZA
dc.publisherWiley Open Access
dc.rights.holderAuthors retain copyright
dc.subjectLeuconostoc speciesen_ZA
dc.subjectDextranen_ZA
dc.subjectLactobacillusen_ZA
dc.subjectLeuconostoc -- South Africaen_ZA
dc.subjectPhylogenetic analysisen_ZA
dc.subjectSugarcane -- Postharvest physiology -- South Africaen_ZA
dc.subjectCladistic analysisen_ZA
dc.titlePhylogenetic analysis of leuconostoc and lactobacillus species isolated from sugarcane processing streamsen_ZA
dc.typeArticleen_ZA
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