Detection of second line drug resistance among drug resistant Mycobacterium tuberculosis isolates in Botswana

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dc.contributor.authorMogashoa, Tueloen_ZA
dc.contributor.authorMelamu, Pinkieen_ZA
dc.contributor.authorDerendinger, Brigittaen_ZA
dc.contributor.authorLey, Serej D.en_ZA
dc.contributor.authorStreicher, Elizabeth M.en_ZA
dc.contributor.authorIketleng, Thatoen_ZA
dc.contributor.authorMupfumi, Lucyen_ZA
dc.contributor.authorMokomane, Margareten_ZA
dc.contributor.authorKgwaadira, Botsheloen_ZA
dc.contributor.authorRankgoane-Pono, Goabaoneen_ZA
dc.contributor.authorTsholofelo, Thusoyaone T.en_ZA
dc.contributor.authorKasvosve, Ishmaelen_ZA
dc.contributor.authorMoyo, Sikhulileen_ZA
dc.contributor.authorWarren, Robin M.en_ZA
dc.contributor.authorGaseitsiwe, Simanien_ZA
dc.date.accessioned2021-10-19T14:23:55Z
dc.date.available2021-10-19T14:23:55Z
dc.date.issued2019-10-28
dc.descriptionCITATION: Mogashoa, T. et al. 2019. Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana. Pathogens, 8(4). doi:10.3390/pathogens8040208
dc.descriptionThe original publication is available at https://www.mdpi.com/journal/pathogens
dc.description.abstractENGLISH ABSTRACT: The emergence and transmission of multidrug resistant (MDR) and extensively drug resistant (XDR) Mycobacterium tuberculosis (M.tb) strains is a threat to global tuberculosis (TB) control. The early detection of drug resistance is critical for patient management. The aim of this study was to determine the proportion of isolates with additional second-line resistance among rifampicin and isoniazid resistant and MDR-TB isolates. A total of 66 M.tb isolates received at the National Tuberculosis Reference Laboratory between March 2012 and October 2013 with resistance to isoniazid, rifampicin or both were analyzed in this study. The genotypes of the M.tb isolates were determined by spoligotyping and second-line drug susceptibility testing was done using the Hain Genotype MTBDRsl line probe assay version 2.0. The treatment outcomes were defined according to the Botswana national and World Health Organization (WHO) guidelines. Of the 57 isolates analyzed, 33 (58%) were MDR-TB, 4 (7%) were additionally resistant to flouroquinolones and 3 (5%) were resistant to both fluoroquinolones and second-line injectable drugs. The most common fluoroquinolone resistance-conferring mutation detected was gyrA A90V. All XDR-TB cases remained smear or culture positive throughout the treatment. Our study findings indicate the importance of monitoring drug resistant TB cases to ensure rapid detection of second-line drug resistance.en_ZA
dc.description.urihttps://www.mdpi.com/2076-0817/8/4/208/htm
dc.description.versionPublisher’s version
dc.format.extent12 pagesen_ZA
dc.identifier.citationMogashoa, T. et al. 2019. Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana. Pathogens, 8(4). doi:10.3390/pathogens8040208
dc.identifier.issn2076-0817 (online)
dc.identifier.otherdoi:10.3390/pathogens8040208
dc.identifier.urihttp://hdl.handle.net/10019.1/123259
dc.language.isoen_ZAen_ZA
dc.publisherMDPIen_ZA
dc.rights.holderAuthors retain rightsen_ZA
dc.subjectMycobacterium tuberculosis -- Botswanaen_ZA
dc.subjectLine probe assayen_ZA
dc.subjectSecond-line drugsen_ZA
dc.subjectDrug resistanceen_ZA
dc.subjectMultidrug-resistant tuberculosisen_ZA
dc.subjectMDR-TBen_ZA
dc.titleDetection of second line drug resistance among drug resistant Mycobacterium tuberculosis isolates in Botswanaen_ZA
dc.typeArticleen_ZA
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