HIV-1C env and gag variation in the cerebrospinal fluid and plasma of patients with HIV-associated cryptococcal meningitis in Botswana

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dc.contributor.authorKelentse, Nametsoen_ZA
dc.contributor.authorMoyo, Sikhulileen_ZA
dc.contributor.authorMogwele, Mompati L.en_ZA
dc.contributor.authorDitshwanelo, Doreenen_ZA
dc.contributor.authorMokaleng, Baitshepien_ZA
dc.contributor.authorMoraka, Natasha O.en_ZA
dc.contributor.authorLechiile, Kwanaen_ZA
dc.contributor.authorLeeme, Tshepo B.en_ZA
dc.contributor.authorLawrence, David S.en_ZA
dc.contributor.authorMusonda, Rosemaryen_ZA
dc.contributor.authorKasvosve, Ishmaelen_ZA
dc.contributor.authorHarrison, Thomas S.en_ZA
dc.contributor.authorJarvis, Joseph N.en_ZA
dc.contributor.authorGaseitsiwe, Simanien_ZA
dc.date.accessioned2021-03-30T09:32:28Z
dc.date.available2021-03-30T09:32:28Z
dc.date.issued2020-12-07
dc.descriptionCITATION: Kelentse, N. et al. 2020. HIV-1C env and gag variation in the cerebrospinal fluid and plasma of patients with HIV-associated cryptococcal meningitis in Botswana. Viruses, 12(12):1404, doi:10.3390/v12121404.
dc.descriptionThe original publication is available at https://www.mdpi.com
dc.description.abstractENGLISH ABSTRACT: HIV-1 compartmentalization in reservoir sites remains a barrier to complete HIV eradication. It is unclear whether there is variation in HIV-1 env and gag between cerebrospinal fluid (CSF) and plasma of individuals with HIV-associated cryptococcal meningitis (CM). We compared HIV-1 env characteristics and the gag cytotoxic T-lymphocyte (CTL) escape mutations from CSF and plasma samples. Employing population-based Sanger sequencing, we sequenced HIV-1 env from CSF of 25 patients and plasma of 26 patients. For gag, 15 CSF and 21 plasma samples were successfully sequenced. Of these, 18 and 9 were paired env and gag CSF/plasma samples, respectively. There was no statistically significant difference in the proportion of CCR5-using strains in the CSF and plasma, (p = 0.50). Discordant CSF/plasma virus co-receptor use was found in 2/18 pairs (11.1%). The polymorphisms in the HIV-1 V3 loop were concordant between the two compartments. From the HIV-1 gag sequences, three pairs had discordant CTL escape mutations in three different epitopes of the nine analyzed. These findings suggest little variation in the HIV-1 env between plasma and CSF and that the CCR5-using strains predominate in both compartments. HIV-1 gag CTL escape mutations also displayed little variation in CSF and plasma suggesting similar CTL selective pressure.en_ZA
dc.description.urihttps://www.mdpi.com/1999-4915/12/12/1404
dc.description.versionPublisher's version
dc.format.extent17 pagesen_ZA
dc.identifier.citationKelentse, N. et al. 2020. HIV-1C env and gag variation in the cerebrospinal fluid and plasma of patients with HIV-associated cryptococcal meningitis in Botswana. Viruses, 12(12):1404, doi:10.3390/v12121404.
dc.identifier.issn1999-4915 (online)
dc.identifier.otherdoi:10.3390/v12121404
dc.identifier.urihttp://hdl.handle.net/10019.1/109728
dc.language.isoen_ZAen_ZA
dc.publisherMDPIen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectHIV infectionsen_ZA
dc.subjectCerebrospinal fluiden_ZA
dc.subjectCryptococcus meningitidisen_ZA
dc.subjectHuman immunodeficiency virusesen_ZA
dc.titleHIV-1C env and gag variation in the cerebrospinal fluid and plasma of patients with HIV-associated cryptococcal meningitis in Botswanaen_ZA
dc.typeArticleen_ZA
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