The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay

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dc.contributor.authorHabte, Habtom H.
dc.contributor.authorDe Beer, Corena
dc.contributor.authorLotz, Zoe E.
dc.contributor.authorTyler, Marilyn G.
dc.contributor.authorSchoeman, Leann
dc.contributor.authorKahn, Delawir
dc.contributor.authorMall, Anwar S.
dc.date.accessioned2010-12-14T12:49:10Z
dc.date.available2010-12-14T12:49:10Z
dc.date.issued2008-05
dc.date.updated2010-11-02T19:14:46Z
dc.description.abstractBackground: The female reproductive tract is amongst the main routes for Human Immunodeficiency Virus (HIV) transmission. Cervical mucus however is known to protect the female reproductive tract from bacterial invasion and fluid loss and regulates and facilitates sperm transport to the upper reproductive tract. The purpose of this study was to purify and characterize pregnancy plug mucins and determine their anti-HIV-1 activity in an HIV inhibition assay. Methods: Pregnancy plug mucins were purified by caesium chloride density-gradient ultra-centrifugation and characterized by Western blotting analysis. The anti-HIV-1 activities of the crude pregnancy plug mucus and purified pregnancy plug mucins was determined by incubating them with HIV-1 prior to infection of the human T lymphoblastoid cell line (CEM SS cells). Results: The pregnancy plug mucus had MUC1, MUC2, MUC5AC and MUC5B. The HIV inhibition assay revealed that while the purified pregnancy plug mucins inhibit HIV-1 activity by approximately 97.5%, the crude pregnancy plug mucus failed to inhibit HIV-1 activity. Conclusion: Although it is not clear why the crude sample did not inhibit HIV-1 activity, it may be that the amount of mucins in the crude pregnancy plug mucus (which contains water, mucins, lipids, nucleic acids, lactoferrin, lysozyme, immunoglobulins and ions), is insufficient to cause viral inhibition or aggregation.en_ZA
dc.description.versionPeer Reviewed
dc.format.extent10 p. :ill.
dc.identifier.citationHabte, HH, De Beer, C, Lotz, ZE, Tyler, MG, Schoeman, L, Kahn, D & Mall, AS 2008, 'The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay', Virology Journal, 5(1):59.en_ZA
dc.identifier.issn1743-422X
dc.identifier.otherhttp://dx.doi.org/10.1186/1743-422X-5-59
dc.identifier.urihttp://hdl.handle.net/10019.1/5111
dc.language.isoen_ZAen_ZA
dc.language.rfc3066en
dc.publisherBioMed Centralen_ZA
dc.rights.holderHabte et al.; licensee BioMed Central Ltd.en_ZA
dc.subjectPregnancy plug mucinsen_ZA
dc.subjectCervix mucusen
dc.subjectAnti-HIV-1 activityen
dc.titleThe inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assayen_ZA
dc.typeArticleen_ZA
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