Glass wool filter preparation of cryopreserved spermatozoa

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dc.contributor.authorCoetzee K.
dc.contributor.authorErasmus E.L.
dc.contributor.authorKruger T.F.
dc.contributor.authorMenkveld R.
dc.contributor.authorLombard C.J.
dc.date.accessioned2011-05-15T16:15:28Z
dc.date.available2011-05-15T16:15:28Z
dc.date.issued1994
dc.description.abstractOligozoospermic and asthenozoospermic semen ejaculates, as well as cryopreserved sperm samples prepared by the wash and swim-up procedure often result in unsatisfactory sperm recovery rates. In this study the glass wool filter and the wash and swim-up preparation procedures were compared on the basis of their 'effective' (number of live sperm per millilitre) recovery rates. The glass wool filter procedure consistantly produced significantly (P = 0.0002) higher viable sperm concentrations, making it the preferred method for the preparation of cryopreserved sperm to be used in assisted reproduction techniques. The use of this preparation procedure has also been shown to have no adverse affect on the fertilizing potential of human spermatozoa in our unit.
dc.description.versionArticle
dc.identifier.citationAndrologia
dc.identifier.citation26
dc.identifier.citation1
dc.identifier.issn03034569
dc.identifier.urihttp://hdl.handle.net/10019.1/13350
dc.subjectarticle
dc.subjectcryopreservation
dc.subjectfertilization
dc.subjecthuman
dc.subjecthuman cell
dc.subjectspermatozoon
dc.subjectComparative Study
dc.subjectCryopreservation
dc.subjectFemale
dc.subjectGlass
dc.subjectHuman
dc.subjectMale
dc.subjectOligospermia
dc.subjectSemen Preservation
dc.subjectSperm Motility
dc.subjectSperm-Ovum Interactions
dc.subjectSpermatozoa
dc.titleGlass wool filter preparation of cryopreserved spermatozoa
dc.typeArticle
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