Correlation between p53 gene mutation, p53 protein labeling and PCNA expression in oral squamous cell carcinomas

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dc.contributor.authorVan Heerden W.F.P.
dc.contributor.authorVan Rensburg E.J.
dc.contributor.authorHemmer J.
dc.contributor.authorRaubenheimer E.J.
dc.contributor.authorEngelbrecht S.
dc.date.accessioned2011-05-15T16:15:33Z
dc.date.available2011-05-15T16:15:33Z
dc.date.issued1998
dc.description.abstractBackground: The prevalence of oral squamous cell carcinoma (OSCC) among the Black community in South Africa is unacceptably high. The association between p53 protein, and PCNA overexpression and the presence of p53 gene mutations was evaluated. Materials and Methods: One hundred and ten formalin-fixed, paraffin-embedded blocks of OSCC were selected for immunohistochemical studies for p53 protein and PCNA expression using the DO-7 and PC10 monoclonal antibodies, respectively. DNA was extracted from fifty-five blocks and exons 5 to 9 of the p53 gene were amplified with nested primers, thereafter sequencing was performed to confirm the presence of mutations detected by single stranded conformational polymorphism. Results: Fifty-six cases (51%) showed p53 expression, while fourteen mutations (25%) were detected. A significant difference was found between the PCNA index in p53 positive and p53 negative tumors while the mean PCNA index for the tumors with p53 mutations was not significantly different from the tumors without mutations. Conclusions: No association between p53 protein overexpression and p53 gene mutations could be demonstrated.
dc.description.versionArticle
dc.identifier.citationAnticancer Research
dc.identifier.citation18
dc.identifier.citation1 A
dc.identifier.issn02507005
dc.identifier.urihttp://hdl.handle.net/10019.1/13386
dc.subjectcycline
dc.subjectmonoclonal antibody
dc.subjectprotein p53
dc.subjectadult
dc.subjectaged
dc.subjectarticle
dc.subjectcancer genetics
dc.subjectdna determination
dc.subjectexon
dc.subjectfemale
dc.subjectframeshift mutation
dc.subjectgene amplification
dc.subjectgene mutation
dc.subjectgene overexpression
dc.subjectgenetic association
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmouth carcinoma
dc.subjectnegro
dc.subjectpriority journal
dc.subjectsingle strand conformation polymorphism
dc.subjectsouth africa
dc.subjectsquamous cell carcinoma
dc.subjectAdult
dc.subjectAfrican Continental Ancestry Group
dc.subjectAged
dc.subjectCarcinoma, Squamous Cell
dc.subjectCell Nucleus
dc.subjectFemale
dc.subjectGenes, p53
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMouth Neoplasms
dc.subjectPoint Mutation
dc.subjectProliferating Cell Nuclear Antigen
dc.subjectSequence Deletion
dc.subjectTumor Suppressor Protein p53
dc.titleCorrelation between p53 gene mutation, p53 protein labeling and PCNA expression in oral squamous cell carcinomas
dc.typeArticle
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