Heterorhabditis safricana n. sp. (Rhabditida: Heterorhabditidae) : a new entomopathogenic nematode from South Africa
Date
2008-11
Authors
Malan, Antoinette P.
Nguyen, Khuong B.
De Waal, Jeanne Y.
Tiedt, Louwrence
Journal Title
Journal ISSN
Volume Title
Publisher
Brill Academic Publishers
Abstract
During a survey for entomopathogenic nematodes in the Western Cape Province of South Africa, a new species in the
genus Heterorhabditis was collected from a peach orchard. The nematode was trapped by the insect-baiting technique using last instar
larvae of Galleria mellonella. The infective juvenile of the new species differs from the morphologically closest species, H. marelatus,
in shorter body length 600 (550-676) vs 685 (588-700) μm and the shorter tail of 93 (86-108) vs 107 (99-117) μm. It differs from all
other species in the vulva pattern of hermaphroditic female. The genital papillae of the male H. safricana n. sp. are typical for species in
the megidis-group (three papillae in terminal group). The average length of the gubernaculum is longer than that of all other species (24
vs 19-23 μm) and the gubernaculum length as a percentage of spicule length (53.9) is less than that of H. mexicana (56), similar to that
of H. floridensis (53.8), but larger than that of all others (51 or less). For molecular characterisation, the species closest to H. safricana
n. sp. is H. marelatus. The length of the ITS rDNA sequence of the new species is characterised by 995 base pairs, identical to that
of H. marelatus, but differs from this species by 25 aligned positions, seven of which are unambiguous autapomorphies. Phylogenetic
trees show further evidence of a separate species status for H. safricana n. sp.
Description
CITATION: Malan, A. P. et al. 2008. Heterorhabditis safricana n. sp. (Rhabditida: Heterorhabditidae) : a new entomopathogenic nematode from South Africa. Nematology, 10(3):381-396, doi:10.1163/156854108783900258.
The original publication is available at http://booksandjournals.brillonline.com/content/journals/10.1163/156854108783900258
The original publication is available at http://booksandjournals.brillonline.com/content/journals/10.1163/156854108783900258
Keywords
Entomopathogenic nematodes -- South Africa, Heterorhabditidae -- South Africa
Citation
Malan, A. P. et al. 2008. Heterorhabditis safricana n. sp. (Rhabditida: Heterorhabditidae) : a new entomopathogenic nematode from South Africa. Nematology, 10(3):381-396, doi:10.1163/156854108783900258.