Sutherlandia frutescens may exacerbate HIV-associated neuroinflammation

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dc.contributor.authorAfrica, Luan Daneen_ZA
dc.contributor.authorSmith, Carineen_ZA
dc.date.accessioned2017-01-18T06:20:02Z
dc.date.available2017-01-18T06:20:02Z
dc.date.issued2015-07-18
dc.date.updated2016-12-09T10:08:14Z
dc.descriptionCITATION: Africa, L. D. & Smith, C. 2015. Sutherlandia frutescens may exacerbate HIV-associated neuroinflammation. Journal of Negative Results in BioMedicine., 14:14, doi:10.1186/s12952-015-0031-y.
dc.descriptionThe original publication is available at https://jnrbm.biomedcentral.com
dc.description.abstractBackground: Neuroinflammation is central to the aetiology of HIV-associated neurocognitive disorders (HAND) that are prevalent in late stage AIDS. Anti-retroviral (ARV) treatments are rolled out relatively late in the context of neuroinflammatory changes, so that their usefulness in directly preventing HAND is probably limited. It is common practice for HIV+ individuals in developing countries to make use of traditional medicines. One such medicine is Sutherlandia frutescens - commonly consumed as a water infusion. Here its efficacy as an anti-inflammatory modality in this context was investigated in an in vitro co-culture model of the blood–brain barrier (BBB). Methods: Single cultures of human astrocytes (HA), HUVECs and primary human monocytes, as well as co-cultures (BBB), were stimulated with HIV-1 subtype B & C Tat protein and/or HL2/3 cell secretory proteins after pre-treatment with S.frutescens extract. Effects of this pre-treatment on pro-inflammatory cytokine secretion and monocyte migration across the BBB were assessed. Results: In accordance with others, B Tat was more pro-inflammatory than C Tat, validating our model. S.frutescens decreased IL-1β secretion significantly (P < 0.0001), but exacerbated both monocyte chemoattractant protein-1 (P < 0001) – a major role player in HIV-associated neuroinflammation – and CD14+ monocyte infiltration across the BBB (P < 0.01). Conclusions: Current data illustrates that the combined use of HL2/3 cells and the simulated BBB presents an accurate, physiologically relevant in vitro model with which to study neuroinflammation in the context of HIV/AIDS. In addition, our results caution against the use of S.frutescens as anti-inflammatory modality at any stage post-HIV infection.en_ZA
dc.description.urihttps://jnrbm.biomedcentral.com/articles/10.1186/s12952-015-0031-y
dc.description.versionPublisher's version
dc.format.extent9 pages
dc.identifier.citationAfrica, L. D. & Smith, C. 2015. Sutherlandia frutescens may exacerbate HIV-associated neuroinflammation. Journal of Negative Results in BioMedicine., 14:14, doi:10.1186/s12952-015-0031-y
dc.identifier.issn1477-5751 (online)
dc.identifier.issn1477-5751 (print)
dc.identifier.otherdoi:10.1186/s12952-015-0031-y
dc.identifier.urihttp://hdl.handle.net/10019.1/100480
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Central
dc.rights.holderAuthors retain copyright
dc.subjectHIV infectionsen_ZA
dc.subjectInflammationen_ZA
dc.subjectSutherlandia frutescensen_ZA
dc.subjectBlood-brain barrieren_ZA
dc.titleSutherlandia frutescens may exacerbate HIV-associated neuroinflammationen_ZA
dc.typeArticle
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