Host urine immunological biomarkers as potential candidates for the diagnosis of tuberculosis

Simple item page
dc.contributor.authorEribo, Osagie A.en_ZA
dc.contributor.authorLeqheka, Monkoe S.en_ZA
dc.contributor.authorMalherbe, Stephanus T.en_ZA
dc.date.accessioned2022-09-13T13:41:07Zen_ZA
dc.date.available2022-09-13T13:41:07Zen_ZA
dc.date.issued2020-10-01en_ZA
dc.descriptionCITATION: Eribo, O. A. et al. 2020. Host urine immunological biomarkers as potential candidates for the diagnosis of tuberculosis. International Journal of Inclusive Education, 99:473-481. doi:10.1080/13603116.2019.1619850en_ZA
dc.descriptionThe original publication is available at https://www.journals.elsevier.com/international-journal-of-infectious-diseasesen_ZA
dc.description.abstractObjective: To investigate the potential of host urinary biomarkers as diagnostic candidates for tuberculosis (TB). Methods: Adults self-presenting with symptoms requiring further investigation for TB were enrolled in Cape Town, South Africa. Participants were later classified as having TB or other respiratory diseases (ORD) using results from TB confirmatory tests. The concentrations of 29 analytes were evaluated in urine samples from participants using the Luminex platform, and their diagnostic potential was assessed using standard statistical approaches. Results: Of the 151 study participants, 34 (22.5%) were diagnosed with TB and 26 (17.2%) were HIV-positive. Seven biomarkers showed potential as TB diagnostic candidates, with accuracy improving (in HIV-positives) when stratified according to HIV status (area under the receiver operating characteristics curve; AUC ≥0.80). In HIV-positive participants, a four-marker biosignature (sIL6R, MMP-9, IL-2Ra, IFN-γ) diagnosed TB with AUC of 0.96, sensitivity of 85.7% (95% confidence interval (CI) 42.1-99.6%), and specificity of 94.7% (95% CI 74.0-99.9%). In HIV-negatives, the most promising was a two-marker biosignature (sIL6R and sIL-2Ra), which diagnosed TB with AUC of 0.76, sensitivity of 53.9% (95% CI 33.4-73.4%), and specificity of 79.6% (95% CI 70.3-87.1%). Conclusions: Urinary host inflammatory biomarkers possess TB diagnostic potential but may be influenced by HIV infection. The results of this study require validation in larger studies. Keywords: Biomarker; Biosignature; Diagnosis; Inflammatory biomarker; Tuberculosis; Urine.en_ZA
dc.description.urihttps://www.journals.elsevier.com/international-journal-of-infectious-diseasesen_ZA
dc.description.versionPublishers versionen_ZA
dc.format.extent9 pagesen_ZA
dc.identifier.citationEribo, O. A. et al. 2020. Host urine immunological biomarkers as potential candidates for the diagnosis of tuberculosis. International Journal of Inclusive Education, 99:473-481. doi:10.1080/13603116.2019.1619850en_ZA
dc.identifier.issn1878-3511 (online)en_ZA
dc.identifier.issn1201-9712 (print)en_ZA
dc.identifier.otherdoi:10.1080/13603116.2019.1619850en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/125717en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherElsevieren_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectTuberculosis -- Diagnosisen_ZA
dc.subjectBiochemical markersen_ZA
dc.subjectTuberculosis -- Mortalityen_ZA
dc.subjectRespiratory organs -- Diseasesen_ZA
dc.subjectUrinary biomarkersen_ZA
dc.subjectImmunodiagnosisen_ZA
dc.titleHost urine immunological biomarkers as potential candidates for the diagnosis of tuberculosisen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
eribo_host_2020.pdf
Size:
1.65 MB
Format:
Adobe Portable Document Format
Description:
Download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Molecular Biology and Human Genetics)