HIV type 1 V3 domain serotyping and genotyping in Gauteng, Mpumalanga, Kwazulu-Natal, and Western Cape Provinces of South Africa

Simple item page
dc.contributor.authorEngelbrecht S.
dc.contributor.authorSmith T.-L.
dc.contributor.authorKasper P.
dc.contributor.authorFaatz E.
dc.contributor.authorZeier M.
dc.contributor.authorMoodley D.
dc.contributor.authorClay C.G.
dc.contributor.authorVan Rensburg E.J.
dc.date.accessioned2011-05-15T16:15:16Z
dc.date.available2011-05-15T16:15:16Z
dc.date.issued1999
dc.description.abstractMore than 20.8 million people are living with HIV/AIDS in sub-Saharan Africa, with southern Africa the worst affected area and accounting for one of the fastest growing AIDS epidemics worldwide. Samples from 81 patients, including 25 from KwaZulu-Natal, 26 from Gauteng, 5 from Mpumalanga, and 25 from Western Cape Province, were serotyped using a competitive V3 peptide enzyme immunoassay (cPEIA). Viral RNA was also isolated from serum and the V3 region amplified by reverse transcriptase polymerase chain reaction (RTPCR) to obtain a 240-bp product for direct sequencing of 29 samples. CLUSTAL W was used to make multiple sequence alignments. Distance calculation, tree construction methods, and bootstrap analysis were done using TREECON. Subtype C-like V3 100p sequences predominate in all provinces tested in South Africa. Discordant sero- and genotype results were observed in one patient only. The correlation between sero- and genotyping was 96% (24 of 25) in KwaZulu-Natal and 100% in Gauteng and Mpumalanga. In Western Cape Province 18% of patients were identified as sero/genotype B and 82% as sero/genotype C. Our data show that results of the second-generation V3 cPEIA correlated well with V3 sequencing and would be a rapid and affordable screening test to monitor the explosive southern African HIV-1 epidemic.
dc.description.versionArticle
dc.identifier.citationAIDS Research and Human Retroviruses
dc.identifier.citation15
dc.identifier.citation4
dc.identifier.issn08892229
dc.identifier.other10.1089/088922299311286
dc.identifier.urihttp://hdl.handle.net/10019.1/13265
dc.subjectvirus RNA
dc.subjectacquired immune deficiency syndrome
dc.subjectarticle
dc.subjectgenotype
dc.subjecthuman immunodeficiency virus 1
dc.subjecthuman immunodeficiency virus infection
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpriority journal
dc.subjectreverse transcription polymerase chain reaction
dc.subjectscreening test
dc.subjectserotype
dc.subjectSouth Africa
dc.subjectvirus infection
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAged
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectDNA, Viral
dc.subjectGenotype
dc.subjectHIV Envelope Protein gp120
dc.subjectHIV Infections
dc.subjectHIV-1
dc.subjectHumans
dc.subjectMiddle Aged
dc.subjectMolecular Sequence Data
dc.subjectPeptide Fragments
dc.subjectPhylogeny
dc.subjectSerotyping
dc.subjectSouth Africa
dc.titleHIV type 1 V3 domain serotyping and genotyping in Gauteng, Mpumalanga, Kwazulu-Natal, and Western Cape Provinces of South Africa
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Tygerberg Hospital Publications