Qualitative and quantitative detection of SARS-CoV-2 RNA in untreated wastewater in Western Cape Province, South Africa

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dc.contributor.authorJohnson, R.en_ZA
dc.contributor.authorMuller, C. J. F.en_ZA
dc.contributor.authorGhoor, S.en_ZA
dc.contributor.authorLouw, J.en_ZA
dc.contributor.authorArcher, E.en_ZA
dc.contributor.authorSurujlal-Naicker, S.en_ZA
dc.contributor.authorBerkowitz, N.en_ZA
dc.contributor.authorVolschenk, M.en_ZA
dc.contributor.authorBrocker, L. H. L.en_ZA
dc.contributor.authorWolfaardt, G.en_ZA
dc.contributor.authorVan der Walt, M.en_ZA
dc.contributor.authorMutshembele, A. M.en_ZA
dc.contributor.authorMalema, S.en_ZA
dc.contributor.authorGelderblom, H. C.en_ZA
dc.contributor.authorMuhdluli, M.en_ZA
dc.contributor.authorGray, G.en_ZA
dc.contributor.authorMathee, A.en_ZA
dc.contributor.authorStreet, R.en_ZA
dc.date.accessioned2021-07-14T05:46:36Z
dc.date.available2021-07-14T05:46:36Z
dc.date.issued2021-01-28
dc.descriptionCITATION: Johnson, R. et al. 2021. Qualitative and quantitative detection of SARS-CoV-2 RNA in untreated wastewater in Western Cape Province, South Africa. South African Medical Journal, 111(3):198-202, doi:10.7196/SAMJ.2021.v111i3.15154.
dc.descriptionThe original publication is available at http://www.samj.org.za
dc.description.abstractENGLISH ABSTRACT: Recent studies have shown that the detection of SARS-CoV-2 genetic material in wastewater may provide the basis for a surveillance system to track the environmental dissemination of this virus in communities. An effective wastewater-based epidemiology (WBE) system may prove critical in South Africa (SA), where health systems infrastructure, testing capacity, personal protective equipment and human resource capacity are constrained. In this proof-of-concept study, we investigated the potential of SARS-CoV-2 RNA surveillance in untreated wastewater as the basis for a system to monitor COVID-19 prevalence in the population, an early warning system for increased transmission, and a monitoring system to assess the effectiveness of interventions. The laboratory confirmed the presence (qualitative analysis) and determined the RNA copy number of SARS-CoV-2 viral RNA by reverse transcription polymerase chain reaction (quantitative) analysis from 24-hour composite samples collected on 18 June 2020 from five wastewater treatment plants in Western Cape Province, SA. The study has shown that a WBE system for monitoring the status and trends of COVID-19 mass infection in SA is viable, and its development and implementation may facilitate the rapid identification of hotspots for evidence-informed interventions.en_ZA
dc.description.urihttp://www.samj.org.za/index.php/samj/article/view/13190
dc.description.versionPublisher's version
dc.format.extent5 pagesen_ZA
dc.identifier.citationJohnson, R. et al. 2021. Qualitative and quantitative detection of SARS-CoV-2 RNA in untreated wastewater in Western Cape Province, South Africa. South African Medical Journal, 111(3):198-202, doi:10.7196/SAMJ.2021.v111i3.15154.
dc.identifier.issn2078-5135 (online)
dc.identifier.issn0256-9574 (print)
dc.identifier.otherdoi:10.7196/SAMJ.2021.v111i3.15154
dc.identifier.urihttp://hdl.handle.net/10019.1/110720
dc.language.isoen_ZAen_ZA
dc.publisherHealth & Medical Publishing Groupen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectCOVID-19 (Disease)en_ZA
dc.subjectCoronavirus infections -- Detectionen_ZA
dc.subjectSARS-CoV-2 diseaseen_ZA
dc.subjectWastewater-based epidemiologyen_ZA
dc.subjectPathogensen_ZA
dc.subjectPublic health surveillanceen_ZA
dc.titleQualitative and quantitative detection of SARS-CoV-2 RNA in untreated wastewater in Western Cape Province, South Africaen_ZA
dc.typeArticleen_ZA
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