Identification of novel host biomarkers in plasma as candidates for the immunodiagnosis of tuberculosis disease and monitoring of tuberculosis treatment response

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dc.contributor.authorJacobs, Ruschcaen_ZA
dc.contributor.authorMalherbe, Stephanusen_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.contributor.authorStanley, Kimen_ZA
dc.contributor.authorVan Der Spuy, Gianen_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorChegou, Novel N.en_ZA
dc.date.accessioned2016-09-01T06:56:46Z
dc.date.available2016-09-01T06:56:46Z
dc.date.issued2016-08-19
dc.descriptionCITATION: Jacobs, R., et al. 2016. Identification of novel host biomarkers in plasma as candidates for the immunodiagnosis of tuberculosis disease and monitoring of tuberculosis treatment response. Oncotarget, 7(35):1-12, doi:10.18632/oncotarget.11420.
dc.descriptionThe original publication is available at http://www.impactjournals.com/oncotarget
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.
dc.description.abstractENGLISH ABSTRACT: There is an urgent need for new tools for the rapid diagnosis of tuberculosis disease. We evaluated the potentials of 74 host markers as biomarkers for the immunological diagnosis of tuberculosis and monitoring of treatment response. Fifty-five individuals that presented with signs and symptoms requiring investigation for tuberculosis disease were prospectively recruited prior to clinical diagnosis, at a health centre in Cape Town, South Africa. Patients were later classified as having tuberculosis disease or other respiratory diseases (ORD) using a combination of clinical, radiological and laboratory findings. Out of 74 host markers that were evaluated in plasma samples from study participants using a multiplex platform, 18 showed potential as tuberculosis diagnostic candidates with the most promising being NCAM, CRP, SAP, IP-10, ferritin, TPA, I-309, and MIG, which diagnosed tuberculosis disease individually, with area under the ROC curve ≥0.80. Six-marker biosignatures containing NCAM diagnosed tuberculosis disease with a sensitivity of 100% (95%CI, 86.3-100%) and specificity of 89.3% (95%CI, 67.6-97.3%) irrespective of HIV status, and 100% accuracy in the absence of HIV infection. Furthermore, the concentrations of 11 of these proteins changed with treatment, thereby indicating that they may be useful in monitoring of the response to tuberculosis treatment. Our findings have potential to be translated into a point-of-care screening test for tuberculosis, after future validation studies.en_ZA
dc.description.urihttp://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5B%5D=11420&path%5B%5D=36165
dc.description.versionPublisher's version
dc.format.extent12 pages : illustrationsen_ZA
dc.identifier.citationJacobs, R., et al. 2016. Identification of novel host biomarkers in plasma as candidates for the immunodiagnosis of tuberculosis disease and monitoring of tuberculosis treatment response. Oncotarget, 7(35):1-12, doi:10.18632/oncotarget.11420
dc.identifier.issn1949-2553 (online)
dc.identifier.otherdoi:10.18632/oncotarget.11420
dc.identifier.urihttp://hdl.handle.net/10019.1/99526
dc.language.isoen_ZAen_ZA
dc.publisherImpact Journalsen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectImmunodiagnosis -- Tuberculosisen_ZA
dc.subjectBiomarkeren_ZA
dc.subjectNeural cell adhesion moleculeen_ZA
dc.subjectAcute phase proteinsen_ZA
dc.subjectTuberculosis -- Diagnosisen_ZA
dc.subjectImmune responseen_ZA
dc.subjectTuberculosis -- Chemotherapy -- Monitoringen_ZA
dc.titleIdentification of novel host biomarkers in plasma as candidates for the immunodiagnosis of tuberculosis disease and monitoring of tuberculosis treatment responseen_ZA
dc.typeArticleen_ZA
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