A combination of an antimitotic and a bromodomain 4 inhibitor synergistically inhibits the metastatic MDA-MB-231 breast cancer cell line

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dc.contributor.authorMqoco, Thandien_ZA
dc.contributor.authorStander, Andreen_ZA
dc.contributor.authorEngelbrecht, Anna-Marten_ZA
dc.contributor.authorJoubert, Anna M.en_ZA
dc.date.accessioned2021-08-17T06:54:31Z
dc.date.available2021-08-17T06:54:31Z
dc.date.issued2019-12-11
dc.descriptionCITATION: Mqoco, T., et al. 2019. A combination of an antimitotic and a bromodomain 4 inhibitor synergistically inhibits the metastatic MDA-MB-231 breast cancer cell line. BioMed Research International, 2019(1850462):1-13. doi:10.1155/2019/1850462
dc.descriptionThe original publication is available at https://www.hindawi.com/journals/bmri/
dc.description.abstractCurrent chemotherapeutic agents have many side effects and are toxic to normal cells, providing impetus to identify agents that can effectively eliminate tumorigenic cells without damaging healthy cells. The aim of this study was to examine whether combining a novel BRD4 inhibitor, ITH-47, with the antimitotic estradiol analogue, ESE-15-ol, would have a synergistic effect on inhibiting the growth of two different breast cancer cell lines in vitro. Our docking and molecular dynamics studies showed that compared to JQ1, ITH-47 showed a similar binding mode with hydrogen bonds forming between the ligand nitrogens of the pyrazole, ASN99, and water of the BRD4 protein. Data from cell growth studies revealed that the GI50 of ITH-47 and ESE-15-ol after 48 hours of exposure was determined to be 15 μM and 70 nM, respectively, in metastatic MDA-MB-231 breast cancer cells. In tumorigenic MCF-7 breast cancer cells, the GI50 of ITH-47 and ESE-15-ol was 75 μM and 60 nM, respectively, after 48 hours of exposure. Furthermore, the combination of 7.5 μM and 14 nM of ITH-47 and ESE-15-ol, respectively, resulted in 50% growth inhibition of MDA-MB-231 cells resulting in a synergistic combination index (CI) of 0.7. Flow cytometry studies revealed that, compared to the control, combination-treated MDA-MB-231 cells had significantly more cells present in the sub-G1 phase and the combination treatment induced apoptosis in the MDA-MB-231 cells. Compared to vehicle-treated cells, the combination-treated cells showed decreased levels of the BRD4, as well as c-Myc protein after 48 hours of exposure. In combination, the selective BRD4 inhibitor, ITH-47, and ESE-15-ol synergistically inhibited the growth of MDA-MB-231 breast cancer cells, but not of the MCF-7 cell line. This study provides evidence that resistance to BRD4 inhibitors may be overcome by combining inhibitors with other compounds, which may have treatment potential for hormone-independent breast cancers.en_ZA
dc.description.sponsorshipNRF Thuthuka grant
dc.description.sponsorshipCancer Association of South Africa
dc.description.sponsorshipMedical Research Council
dc.description.sponsorshipSchool of Medicine Research Committee
dc.description.sponsorshipStruwig Germeshuysen Trust
dc.description.urihttps://www.hindawi.com/journals/bmri/2019/1850462/
dc.description.versionPublisher’s version
dc.format.extent14 pages : illustrations (some color)en_ZA
dc.identifier.citationMqoco, T., et al. 2019. A combination of an antimitotic and a bromodomain 4 inhibitor synergistically inhibits the metastatic MDA-MB-231 breast cancer cell line. BioMed Research International, 2019(1850462):1-13. doi:10.1155/2019/1850462
dc.identifier.issn2314-6141 (online)
dc.identifier.issn2314-6133 (print)
dc.identifier.otherdoi:10.1155/2019/1850462
dc.identifier.urihttp://hdl.handle.net/10019.1/110858
dc.language.isoen_ZAen_ZA
dc.publisherHindawi Publishing
dc.rights.holderAuthors retain copyright
dc.subjectBreast -- Cancer -- Chemotherapyen_ZA
dc.subjectBromodomain-containing protein 4 -- Inhibitorsen_ZA
dc.subjectCancer cells -- Growthen_ZA
dc.subjectAntimitotic -- Inhibitorsen_ZA
dc.titleA combination of an antimitotic and a bromodomain 4 inhibitor synergistically inhibits the metastatic MDA-MB-231 breast cancer cell lineen_ZA
dc.typeArticleen_ZA
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