Stable isotope markers differentiate between mass-reared and wild Lepidoptera in sterile insect technique programs

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dc.contributor.authorHood-Nowotny, Rebeccaen_ZA
dc.contributor.authorHarari, Allyen_ZA
dc.contributor.authorSeth, Rakesh K.en_ZA
dc.contributor.authorWee, Suk Lingen_ZA
dc.contributor.authorConlong, Des E.en_ZA
dc.contributor.authorSuckling, David M.en_ZA
dc.contributor.authorWoods, Billen_ZA
dc.contributor.authorLebdi-Grissa, Kaoutharen_ZA
dc.contributor.authorSimmons, Gregoryen_ZA
dc.contributor.authorCarpenter, James E.en_ZA
dc.date.accessioned2017-08-10T13:16:46Z
dc.date.available2017-08-10T13:16:46Z
dc.date.issued2016
dc.descriptionCITATION: Hood-Nowotny, R., et al. 2016. Stable isotope markers differentiate between mass-reared and wild Lepidoptera in sterile insect technique programs. Florida Entomologist, 99(1):Florida Entomologist, 99(1):166-176.en_ZA
dc.descriptionThe original publication is available at http://journals.fcla.edu/flaenten_ZA
dc.description.abstractIn this study we identified a number of moth (Lepidoptera) species that are potential targets for the sterile insect technique (SIT), and we assessed the feasibility of using stable isotope signatures as markers to distinguish mass-reared from wild moth species. Large natural differences in the isotopic signatures of commercially available sugars render them novel markers for mass-reared insects. Sugar beet (Beta vulgaris L.; Caryophyllales: Amaranthaceae), a C3 plant, has a stable isotopic signature (a measure of the ratio of the stable isotopes 13C:12C) of around −27‰ relative to Vienna Pee Dee Belemnite (VPDB; the international C isotope standard for the stable isotopes, 13C and 12C), and sugarcane (Saccharum spp.; Poales: Poaceae), a C4 plant, has an isotopic signature of around −11‰. Thus by means of such a distinct isotope ratio in the sugar in the diet, mass-reared insects can be easily distinguished from wild insects with a high degree of certainty. It was shown that the method could be extended using a multiple isotope approach, with 15N or a full suite of C, N, S and O isotopes. Intrinsic isotope marking of mass-reared moths proved to be an accurate means of distinguishing wild from mass-reared populations, based on isotopic differences between the wild host plant species and the diets used in mass-rearing, which where possible, had been manipulated to contain the isotopically divergent sugar type. This intrinsic labeling using stable isotopes could be useful in the assessment of the quality of mass-reared moths, because a stable isotope is a marker that does not affect the insect in any detrimental manner.en_ZA
dc.description.urihttp://journals.fcla.edu/flaent/article/view/88498
dc.description.versionPublisher's versionen_ZA
dc.format.extent11 pages : illustrationsen_ZA
dc.identifier.citationHood-Nowotny, R., et al. 2016. Stable isotope markers differentiate between mass-reared and wild Lepidoptera in sterile insect technique programs. Florida Entomologist, 99(1):Florida Entomologist, 99(1):166-176en_ZA
dc.identifier.issn0015-4040 (online)
dc.identifier.urihttp://hdl.handle.net/10019.1/102084
dc.language.isoen_ZAen_ZA
dc.publisherFlorida Entomological Societyen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectSterile insect technique (SIT)en_ZA
dc.subjectMoth (Lepidoptera) speciesen_ZA
dc.subjectMass-reared moth speciesen_ZA
dc.subjectWild moth speciesen_ZA
dc.titleStable isotope markers differentiate between mass-reared and wild Lepidoptera in sterile insect technique programsen_ZA
dc.typeArticleen_ZA
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