Comparison of the permeability of vaginal mucosa and the wall of an experimental cyst
Date
2000
Journal Title
Journal ISSN
Volume Title
Publisher
Academy of Science of South Africa
Abstract
We used human vaginal mucosa to establish an experimental
non-keratinized cyst in immunodeficient mice' and demonstrated
that It could be used as a blotest mOdel.2 Because the histology of
vaginal and buccal mucosa is broadly similar and the permeability
of vaginal epithelium to watar and 17~-eatradiolls comparable to
that of buccal mucosa,3,4 we concluded that these features Indicate
that the cyst could also be used for Investigating the pathogenesis
of various oral mucosal pathosas. The aim of the present study,
therefore, was to compare the permeability of the epithelium lining
the experimental cyst with that of fresh vaginal epithelium. Fresh
vaginal mucosa was obtained from six postmenopausal women
who had undergone reconstructive vaginal surgery. Seven tiHue
disks were prepared from each of the vaginal specimens, mounted
In flow-through diffusion cells and their permeability to tritiated
water determined as previously described.3 Six artificial cysts were
constructed from an additional six specimens, Implanted in nude
mice and harvested after nine weeks. Seven tissue disks were
Immediately prepared from each of the cysts, mounted In
flow-through diffusion cells and their permeability to tritiated water
determined. No statistical differences (P > 0.05) (Kruskal-Wallls
test) could be demonstrated between the flux values of the vaginal
and experimental cyst epithelium. The similar permeability characteristics of the vaginal and cyst epithelium to water lend support to
the hypothesis that the experimental cyst developed from vaginal
mucosa can be considered as a substitute model for studies on the
buccal mucosa.
Description
CITATION: Thompson, I. O. C. et al. 2000. Comparison of the permeability of vaginal mucosa and the wall of an experimental cyst. South African Journal of Science, 96:385-390.
The original publication is available at https://journals.co.za/doi/abs/10.10520/AJA00382353_8947
The original publication is available at https://journals.co.za/doi/abs/10.10520/AJA00382353_8947
Keywords
Citation
Thompson, I. O. C. et al. 2000. Comparison of the permeability of vaginal mucosa and the wall of an experimental cyst. South African Journal of Science, 96:385-390.