Epigenetics and proteomics join transcriptomics in the quest for tuberculosis biomarkers

Simple item page
dc.contributor.authorEsterhuyse, Maria M.en_ZA
dc.contributor.authorWeiner, Januaryen_ZA
dc.contributor.authorCaron, Etienneen_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.contributor.authorIannaccone, Marcoen_ZA
dc.contributor.authorWagman, Chandreen_ZA
dc.contributor.authorSaikali, Philippeen_ZA
dc.contributor.authorStanley, Kimen_ZA
dc.contributor.authorWolski, Witold E.en_ZA
dc.contributor.authorMollenkopf, Hans-Joachimen_ZA
dc.contributor.authorSchick, Matthiasen_ZA
dc.contributor.authorAebersold, Ruedien_ZA
dc.contributor.authorLinhart, Heinzen_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorKaufmann, Stefan H. E.en_ZA
dc.date.accessioned2016-11-02T13:45:16Z
dc.date.available2016-11-02T13:45:16Z
dc.date.issued2015-09-15
dc.descriptionCITATION: Esterhuyse, M. M. et al. 2015. Epigenetics and proteomics join transcriptomics in the quest for tuberculosis biomarkers. mBio, 6(5):e01187-15, doi:10.1128/mBio.01187-15.
dc.descriptionThe original publication is available at http://mbio.asm.org
dc.description.abstractAn estimated one-third of the world’s population is currently latently infected with Mycobacterium tuberculosis. Latent M. tuberculosis infection (LTBI) progresses into active tuberculosis (TB) disease in ~5 to 10% of infected individuals. Diagnostic and prognostic biomarkers to monitor disease progression are urgently needed to ensure better care for TB patients and to decrease the spread of TB. Biomarker development is primarily based on transcriptomics. Our understanding of biology combined with evolving technical advances in high-throughput techniques led us to investigate the possibility of additional platforms (epigenetics and proteomics) in the quest to (i) understand the biology of the TB host response and (ii) search for multiplatform biosignatures in TB. We engaged in a pilot study to interrogate the DNA methylome, transcriptome, and proteome in selected monocytes and granulocytes from TB patients and healthy LTBI participants. Our study provides first insights into the levels and sources of diversity in the epigenome and proteome among TB patients and LTBI controls, despite limitations due to small sample size. Functionally the differences between the infection phenotypes (LTBI versus active TB) observed in the different platforms were congruent, thereby suggesting regulation of function not only at the transcriptional level but also by DNA methylation and microRNA. Thus, our data argue for the development of a large-scale study of the DNA methylome, with particular attention to study design in accounting for variation based on gender, age, and cell type.en_ZA
dc.description.urihttp://mbio.asm.org/content/6/5/e01187-15.abstract?sid=fe0ea1c7-6da2-4e53-b4a4-5cd8233777c7
dc.description.versionPublisher's version
dc.format.extent13 pages
dc.identifier.citationEsterhuyse, M. M. et al. 2015. Epigenetics and proteomics join transcriptomics in the quest for tuberculosis biomarkers. mBio, 6(5):e01187-15, doi:10.1128/mBio.01187-15.
dc.identifier.issn2150-7511 (online)
dc.identifier.otherdoi:10.1128/mBio.01187-15
dc.identifier.urihttp://hdl.handle.net/10019.1/99817
dc.language.isoen_ZAen_ZA
dc.publisherAmerican Society for Microbiology
dc.rights.holderAuthors retain copyright
dc.subjectTuberculosis Biomarkersen_ZA
dc.subjectEpigeneticsen_ZA
dc.subjectProteomicsen_ZA
dc.subjectTranscriptomicsen_ZA
dc.subjectMycobacterium tuberculosis -- Genetic aspectsen_ZA
dc.subjectBiomarkersen_ZA
dc.subjectBiochemical markersen_ZA
dc.titleEpigenetics and proteomics join transcriptomics in the quest for tuberculosis biomarkersen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
esterhuyse_epigenetics_2015.pdf
Size:
626.65 KB
Format:
Adobe Portable Document Format
Description:
Download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.95 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Molecular Biology and Human Genetics)