Cytomorphological patterns of M. bovis BCG and M. tuberculosis on fine needle aspiration biopsies: Does HIV make a difference?

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dc.contributor.authorSchubert P.T.
dc.contributor.authorCotton M.F.
dc.contributor.authorWright C.A.
dc.date.accessioned2011-05-15T16:16:15Z
dc.date.available2011-05-15T16:16:15Z
dc.date.issued2011
dc.description.abstractTo determine if fine needle aspiration (FNAB) of mycobacterial lymphadenopathy can differentiate infection with M. bovis BCG (BCG) from M. tuberculosis (TB) and whether HIV status affects discriminatory cytological features. A retrospective study of culture positive, fine needle aspiration biopsies of lymph nodes in children (<13 years) between 2003 and 2008. A total of 77 aspirates were available for evaluation with 67 (87%) patients having known HIV status. BCG occurred at a younger age (6 months), predominantly axillary lymph nodes (90%) compared with TB (5 years and 20% axillary lymph nodes). Amorphous necrosis was only seen in aspirates fromTB lymph nodes, while in HIV negative children with TB, foamy macrophages were absent. OnZN staining there were more organisms in the BCG group and in HIV positive patients the organisms were present in both extra- and intracellular locations, whereas in the HIV negative patients the organisms were predominantly extracellular in location. Demographic and cytomorphologic features that can assist in distinguishing between thetwo mycobacterial species include: age of patient, location of the lymph node, and presence/absence of amorphous necrosis and foamy macrophages on FNAB. However the only reliable method to identify the mycobacterial species is by mycobacterial culture and/orPCR. © 2010 Wiley-Liss, Inc.
dc.description.versionArticle
dc.identifier.citationDiagnostic Cytopathology
dc.identifier.citation39
dc.identifier.citation4
dc.identifier.issn87551039
dc.identifier.other10.1002/dc.21378
dc.identifier.urihttp://hdl.handle.net/10019.1/13695
dc.subjectadolescent
dc.subjectage
dc.subjectarticle
dc.subjectaspiration biopsy
dc.subjectbacterium culture
dc.subjectbovine tuberculosis
dc.subjectcell structure
dc.subjectchild
dc.subjectcontrolled study
dc.subjectdemography
dc.subjectextracellular space
dc.subjectfemale
dc.subjectgiant cell
dc.subjectgranuloma
dc.subjecthuman
dc.subjectHuman immunodeficiency virus infection
dc.subjectinfant
dc.subjectintracellular space
dc.subjectlymph node
dc.subjectlymphadenopathy
dc.subjectlymphocyte
dc.subjectmacrophage
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectMycobacterium bovis
dc.subjectMycobacterium tuberculosis
dc.subjectnecrosis
dc.subjectneutrophil
dc.subjectpolymerase chain reaction
dc.subjectpreschool child
dc.subjectpriority journal
dc.subjectretrospective study
dc.subjectschool child
dc.titleCytomorphological patterns of M. bovis BCG and M. tuberculosis on fine needle aspiration biopsies: Does HIV make a difference?
dc.typeArticle
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