Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose

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dc.contributor.advisorBotha, F. C.
dc.contributor.advisorGroenewald, J-H.
dc.contributor.authorOrendo-Smith, R.en_ZA
dc.date.accessioned2006-11-17T07:13:02Zen_ZA
dc.date.accessioned2010-06-01T08:49:21Z
dc.date.available2006-11-17T07:13:02Zen_ZA
dc.date.available2010-06-01T08:49:21Z
dc.date.issued2005-12
dc.descriptionThesisa (MSc (Genetics. Plant Biotechnology))--University of Stellenbosch, 2005.
dc.description.abstractThe study had two main objectives: 1) to investigate specific enzyme activity profiles at various developmental stages and to determine possible implications for sucrose metabolism, 2) to incorporate enzyme activity data of different internodes to obtain a detailed model of every stage in the tissue maturation process. The most significant findings of the regulation of sucrose accumulation in this study are centred on three main point controls in sucrose metabolism pathway. Firstly, the maturation of sugarcane internodes coincided with an increase of SPS in most genotypes, and this underlines the key role of this enzyme in sucrose accumulation. Secondly, SuSy activity (cleavage reaction) correlated negatively with sucrose concentration and hence with tissue maturation process, in most of the varieties. This finding indicates that SuSy could well be implicated in sucrose metabolism. Thirdly, in vitro PFP activity was found to be negatively correlated to sucrose content in sugarcane varieties differing in amount of sucrose.en_ZA
dc.format.extent518854 bytesen_ZA
dc.format.extent139079 bytesen_ZA
dc.format.mimetypeapplication/pdfen_ZA
dc.format.mimetypeapplication/pdfen_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/2462
dc.publisherStellenbosch : University of Stellenbosch
dc.rights.holderUniversity of Stellenbosch
dc.subjectTheses -- Plant biotechnologyen_ZA
dc.subjectDissertations -- Plant biotechnologyen_ZA
dc.subjectSugarcane -- Compositionen
dc.subjectSucrose -- Metabolismen
dc.subjectEnzymesen
dc.subject.otherGeneticsen_ZA
dc.subject.otherInstitute for Plant Biotechnologyen_ZA
dc.titleEnzyme profiling of a range of sugarcane tissue types with different levels of sucroseen_ZA
dc.typeThesisen_ZA
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