Expression of a small ubiquitin-like modifier Protease increases drought tolerance in wheat (Triticum aestivum L.)

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dc.contributor.authorLe Roux, Marlon L.en_ZA
dc.contributor.authorKunert, Karl J.en_ZA
dc.contributor.authorVan der Vyver, Christellen_ZA
dc.contributor.authorCullis, Christopher A.en_ZA
dc.contributor.authorBotha, Anna-Mariaen_ZA
dc.date.accessioned2021-07-06T12:16:12Z
dc.date.available2021-07-06T12:16:12Z
dc.date.issued2019-03-08
dc.descriptionCITATION: Le Roux, M. L. et al. 2019. Expression of a small ubiquitin-like modifier Protease increases drought tolerance in wheat (Triticum aestivum L.). Frontiers in Plant Science, 10:266, doi:10.3389/fpls.2019.00266.
dc.descriptionThe original publication is available at https://www.frontiersin.org
dc.description.abstractPost-translation modification of proteins plays a critical role in cellular signaling processes. In recent years, the SUMO (Small Ubiquitin-Like Modifier) class of molecules has emerged as an influential mechanism for target protein management. SUMO proteases play a vital role in regulating pathway flux and are therefore ideal targets for manipulating stress-responses. In the present study, the expression of an Arabidopsis thaliana cysteine protease (OVERLY TOLERANT TO SALT-1, OTS1) in wheat (Triticum aestivum L.) has led to improved plant growth under water stress conditions. Transformed wheat (pUBI-OTS1) displayed enhanced growth and delayed senescence under water deficit when compared with untransformed Gamtoos-R genotype or plants carrying an empty vector. Transformed pUBI-OTS1 plants also maintained a high relative moisture content (RMC), had a higher photosynthesis rate, and also had a higher total chlorophyll content when compared to untransformed plants or plants carrying an empty vector. SUMOylation of total protein also increased in untransformed plants but not in the AtOTS1 transformed plants. Our results suggest that SUMO-proteases may influence an array of mechanisms in wheat to the advantage of the crop to be more tolerant to water stress caused by drought. This is the first report to elucidate SUMOylation effects in the hexaploid crop wheat (T. aestivum L.).en_ZA
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fpls.2019.00266/full
dc.description.versionPublisher's version
dc.format.extent15 pages : illustrations (some color)en_ZA
dc.identifier.citationLe Roux, M. L. et al. 2019. Expression of a small ubiquitin-like modifier Protease increases drought tolerance in wheat (Triticum aestivum L.). Frontiers in Plant Science, 10:266, doi:10.3389/fpls.2019.00266.
dc.identifier.issn1664-462X (online)
dc.identifier.otherdoi:10.3389/fpls.2019.00266
dc.identifier.urihttp://hdl.handle.net/10019.1/110644
dc.language.isoen_ZAen_ZA
dc.publisherFrontiers
dc.rights.holderAuthors retain copyright
dc.subjectWheat (Triticum aestivum L.)en_ZA
dc.subjectWater -- Effect of global warming onen_ZA
dc.subjectWheat -- Drought toleranceen_ZA
dc.subjectUbiquitinen_ZA
dc.subjectProteaseen_ZA
dc.subjectWheat -- Effect of salt onen_ZA
dc.subjectWheat -- Geneticsen_ZA
dc.titleExpression of a small ubiquitin-like modifier Protease increases drought tolerance in wheat (Triticum aestivum L.)en_ZA
dc.typeArticleen_ZA
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